FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b506.3520000003f846.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b506.3520000003f846.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173258
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA31831.8371446051553175No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTCAAATTCACAAGCAC24461.4117674219949439No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA23041.3298087245610593No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT21981.2686282884484412No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA16650.9609945861085779No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG16000.9234782809451799No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC15570.8986598021447783No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT12810.7393597986817347No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT12770.7370511029793718No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA12700.7330108855002366No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA11670.6735619711643906No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT11360.6556695794710778No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT10170.5869858823257801No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT9660.5575500121206525No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT8660.49983261956157865No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT8370.48309457571944725No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA8140.4698195754308603No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG8070.46577935795172515No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA7090.4092163132438329No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG6260.36131087741980167No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT5640.325526094033176No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA5300.3059021805630909No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA4600.26550000577173927No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4250.24529891837606344No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAAGGCCTGATTCTG4240.2447217444504727No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG3640.21009130891502847No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTATCATAATGGATACTTTTATT2960.1708434819748583No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTATGATGACCTCGATTATTAAAT2890.16680326449572314No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2690.1552597859839084No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA2250.12986413325791593No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA2170.12524674185319004No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT1860.10735435015987718No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA1840.10620000230869571No Hit
GGGGGGAGCAGAAGCGGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA1750.10100543697837906No Hit
AGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGAACTAGA1750.10100543697837906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT20750.0292.156621
CGGGTTA21200.0284.56372
GGGGGGA16500.0281.59091
GTTATTA21750.0277.367835
GGGTTAT22300.0270.526923
TTATTAG23350.0258.99366
TATTAGT24200.0250.506187
ATTAGTA25000.0241.98
TTAGTAG26200.0231.38369
GGGGAGC20400.0227.03433
GGAGCAG20800.0226.213945
GGGGGAG21150.0221.075652
GGTTATT27500.0219.909094
GAGCAGA21650.0216.651286
CAGAAGC22200.0209.954969
GGGAGCA23300.0200.042924
GCAGAAG27250.0171.045878
AGCAGAA30850.0151.08597
CGTAGGT200.0067327926147.5295
ACGTGTG200.0067327926147.5295
Produced by FastQC (version 0.11.2)