FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b49.3520000003f7b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b49.3520000003f7b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340153
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA314599.248485240465321No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA267907.875867624274958No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA66231.9470649972218386No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA54791.6107457526466034No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC48511.4261229505546034No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA45331.3326356080940047No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT42911.261491152510782Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT34761.0218930892862919No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT21960.6455918366146999No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT21750.6394181441880565No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA21570.6341264078223623No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA19940.5862067951774643No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG19790.5817970148727191No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAA15800.46449685876649627No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA11800.3469027173066238No Hit
GGGGGGAGGGAAAAATCTGTGTACTTGTACTGCCGAGTGAATGGCACAAA10120.29751317789347737No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT8070.2372461803952927No Hit
GGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAAA7380.21696119099346473No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA7020.2063777182620762No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA6650.195500260177038No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTTATATTTATTTTAATGAGA6430.189032582396745No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT5970.17550925612885965No Hit
GGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAAAA5940.1746273000679106No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT5650.16610172481206986No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG5630.1655137541047705No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA5370.15787013490987878No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT5350.15728216420257943No Hit
AAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGA5180.15228441319053485No Hit
GAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAG5120.15052050106863674No Hit
GGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAAAAT4660.1369971748007514No Hit
AGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGA4610.13552724803250302No Hit
GCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAAAATAGA4280.12582573136206354No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTATATTTATTTTAATGAGA4150.12200392176461769No Hit
GAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACAAAATA3980.11700617075257312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT54250.0292.009221
CGGGTTA54450.0291.207522
GGGTTAT54950.0288.55783
GGGGGGA61050.0286.060581
GTTATTA55500.0284.635135
TATTAGT56350.0279.556347
GGTTATT56950.0277.647064
ATTAGTA56900.0276.854168
TTAGTAG57050.0275.867659
TTATTAG59650.0264.33786
GGGGAGC65700.0260.87523
GGGGGAG70300.0248.001422
GGAGCAG69850.0244.953465
GAGCAGA71750.0239.08366
CAGAAGC72800.0234.824869
AGCAGAA75550.0226.667767
GGGAGCA75900.0225.622544
GCAGAAG76650.0223.030018
GGGAGGG2850.087.982454
CGCTGCC600.001807787973.74999295
Produced by FastQC (version 0.11.2)