FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b48.3520000003f7a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b48.3520000003f7a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147781
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA49743.36579127222038No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG47583.219629045682462No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA44913.0389562934342034No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA29962.027324216238894No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC29361.986723597756139No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG26241.7756003816458137No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT24821.679512251236627No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTG21561.4589155574803256No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT21511.4555321726067627No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA19771.3377903790067736No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT16731.1320805786941488No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT15451.0454659259309382No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT12330.834342709820613No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAGACAATTAAGTTTAG10800.730811132689588No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA10720.7253977168918873No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA10150.6868271293332702No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA9890.669233527990743No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT8110.5487850264919035No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGCGTTCATTG7880.5332214560735141No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT6900.46690711255168116No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA6890.46623043557696864No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA6610.4472834802850163No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT6250.4229231091953634No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAAT5910.3999160920551356No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT4900.33157171760916493No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT4870.32954168668502715No Hit
GGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAAT4770.3227749169379014No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA4570.3092413774436497No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTGT4440.3004445767723862No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTC3650.24698709577009223No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCACTCAATTGCGTTCATTG3220.21788998585745126No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA3210.21721330888273865No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT2990.20232641543906188No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAGACAATTAAGTTTAG2820.19082290686894796No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA2660.17999607527354666No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT2450.16578585880458246No Hit
GGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATT2330.15766573510803147No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA2070.14007213376550437No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTT1870.12653859427125275No Hit
CCGGGTTATTAGTAGTAACAAGAGCTGACCTGGAGGTTTATGCTTTAACA1830.12383188637240242No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT1770.11977182452412691No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCT1760.11909514754941435No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTC1740.11774179359998918No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAA1680.11368173175171367No Hit
GGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAATTG1670.11300505477700111No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA1580.10691496200458787No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCTC1560.10556160805516271No Hit
AGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGA1560.10556160805516271No Hit
AAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGA1530.10353157713102495No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC1520.10285490015631239No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT27400.0291.231751
CGGGTTA27600.0289.121372
GGGGGGA27550.0286.433751
GTTATTA28300.0281.44885
ATTAGTA28700.0275.98438
TTAGTAG28800.0275.53829
TATTAGT28800.0274.001747
GGGGGAG29800.0263.81712
TTATTAG30800.0258.1256
GGGGAGC30450.0257.216743
GGGTTAT32000.0249.367193
GGAGCAG32100.0243.535835
GGTTATT33100.0241.080054
GAGCAGA33450.0234.588946
CAGAAGC34200.0230.307029
GGGAGCA34700.0225.713264
GCAGAAG35700.0220.217078
AGCAGAA35750.0219.083917
CGGAATT255.54676E-5177.0295
TATGTTT3350.0158.50746295
Produced by FastQC (version 0.11.2)