FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b47.3520000003f797.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b47.3520000003f797.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149502
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA27891.8655268825835105No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA25761.723053872189001No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG25751.7223849848162567No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC23841.5946274966221186No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC15811.0575109363085442No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT13020.8708913593129188No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACATTTATTTTGACAAGA12060.8066781715294778No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT11130.7444716458642694No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT10040.671562922235154No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT9320.6234030313975732No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT9040.6046741849607363No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT8670.5799253521692017No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA6590.4407967786384128No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC6370.42608125643804096No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT6130.41002795949218074No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT6030.4033390857647389No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG5480.3665502802638092No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5390.3605302939091116No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT5020.335781461117577No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA4660.3117015156987866No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3820.25551497638827575No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3700.2474883279153456No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTTAATTGTATTCATTG3230.21605062139636927No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT3090.2066861981779508No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT2910.1946462254685556No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA2870.1919706759775789No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2780.1859506896228813No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA2120.1418041230217656No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTTAATTGTATTCATTG1940.1297641503123704No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACATTTATTTTGACAAGA1910.12775748819413787No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA1880.12575082607590532No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT1820.12173750183944027No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG1670.11170419124827763No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT1640.10969752913004507No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTC1630.10902864175730091No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA1510.10100199328437076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA19250.0289.636382
CCGGGTT19200.0289.62241
TATTAGT20450.0271.91937
GGGTTAT20700.0269.347843
ATTAGTA20800.0268.05298
GGGGGGA15750.0263.15871
TTAGTAG21750.0256.344859
TTATTAG22000.0252.090936
GGGGAGC16450.0250.167193
GGTTATT22600.0245.398224
GAGCAGA17050.0240.498546
GGAGCAG17000.0240.338245
GTTATTA23450.0236.503225
CAGAAGC17350.0236.340069
GGGAGCA19700.0208.14724
GGGGGAG20950.0197.840092
GCAGAAG20950.0197.136038
GCCGAAG150.0028472282196.666678
GCTCGCT150.0028472282196.66667295
AGCAGAA23750.0172.652637
Produced by FastQC (version 0.11.2)