FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b44.3520000003f760.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b44.3520000003f760.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150775
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA42362.8094843309567232No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC34752.304758746476538No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA30962.0533908141270105No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA19691.3059194163488643No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT19661.3039296965677334No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG16081.0664898026861218No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA15901.0545514839993366No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA11240.7454816779970154No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA10710.7103299618637042No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT10190.6758414856574366No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA10130.671862046095175No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT8780.5823246559442878No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT7610.5047255844801858No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA6320.4191676338915603No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG5590.37075111921737686No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG5300.3515171613331122No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA4820.31968164483501904No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT4690.3110595257834522No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT4590.30442712651301607No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA4440.294478527607362No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT4250.2818769689935334No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG3680.24407229315204776No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA3650.2420825733709169No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT3620.2400928535897861No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT3190.21157353672691098No Hit
AAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAGA3150.2089205770187365No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3140.2082573370916929No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT2990.1983087381860388No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA2930.19432929862377715No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA2730.181064500082905No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC2400.15917758249046593No Hit
GAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGAG2380.15785110263637872No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAA2380.15785110263637872No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT2150.14259658431437572No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT2060.13662742497098324No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA1920.12734206599237274No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGCGTTCATTG1910.12667882606532913No Hit
AGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGAACTAGATGA1880.12468910628419831No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC1800.11938318686784943No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTG1770.11739346708671862No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAA1580.10479190847289008No Hit
GGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATG1570.10412866854584645No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTT108.4577466E-4295.02
CCGGGTT17600.0290.809661
CGGGTTA18000.0282.708342
GGGGGGA19950.0281.69171
GTTATTA18250.0278.02745
GGGTTAT19000.0266.276343
TATTAGT19250.0265.883127
ATTAGTA19700.0259.809638
TTAGTAG20300.0253.583729
TTATTAG20750.0245.240976
GGTTATT20850.0242.649894
GGAGCAG23550.0238.004245
GGGGAGC23850.0236.247393
GGGGGAG24350.0232.002062
GAGCAGA24250.0231.134026
CAGAAGC25850.0215.116079
AGCAGAA27050.0207.20897
GCAGAAG27550.0201.842128
CCGGGTA304.656904E-7196.666671
GCCCGGC150.0028472517196.66667295
Produced by FastQC (version 0.11.2)