FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b43.3520000003f754.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b43.3520000003f754.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282380
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA103973.681917982859976No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTTACAAGCAC100713.5664707132233158No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT81022.8691833699270486Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA50411.7851830866208656No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT41291.4622140378213755No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT28591.0124654720589277No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT27640.9788228628089809No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA26390.9345562716906296No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT24760.8768326368722998No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT21950.7773213400382464No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA20740.7344712798356824No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA20700.7330547489198952No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA19070.6753311141015653No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG17190.6087541610595651No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTTAAAACTGAAGCAAATAGGCCAA16290.5768822154543523No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTGT11960.42354274382038387No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA11720.4150435583256605No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTACGTTTATTG11710.4146894255967136No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA11180.3959203909625328No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT10920.38671294000991574No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT7770.2751611303916708No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG7180.25426729938380904No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTACGTTTATTG6940.24576811388908565No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT6870.24328918478645797No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT6650.23549826474962818No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA6320.22381188469438343No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAA5790.20504285006020256No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTAT4940.17494156809972378No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTTAAAACTGAAGCAAATAGGCCAA4600.16290105531553226No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA4590.16254692258658546No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTC4180.14802748069976626No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTTAAAACTGAAGCAAATAGGCCAA4120.1459026843260854No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAGAATTG4030.14271548976556414No Hit
GGGGGGAGCAGAAGCAGAGCCATGGATGAACTCCACAACGAAATACTAGA3590.12713364969190452No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTTAT3250.11509313690771301No Hit
CCGGGTTATTAGTAGAAACACGAGCTGGGAGATTCTTGCACAATTCCTCA3140.11119767688929812No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAGAATTG3040.10765634959983002No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCTC3000.1062398186840428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT39100.0293.11381
GGGGGGA38600.0287.739651
CGGGTTA40200.0283.625642
GTTATTA40500.0279.70375
TATTAGT42950.0263.405127
ATTAGTA43250.0261.91918
TTAGTAG43700.0260.234569
GGGTTAT44050.0258.166873
GGGGAGC44100.0250.181413
TTATTAG46650.0242.829596
GGGGGAG47050.0235.74922
GGTTATT49250.0230.609134
GGAGCAG48350.0228.800425
GAGCAGA49550.0222.663976
CAGAAGC50850.0216.681439
AGCAGAA52000.0211.605777
GCAGAAG53150.0206.472248
GGGAGCA59150.0187.024524
GCTCGCT301.1481669E-4147.49998295
CGGGTTT555.9088052E-8134.090912
Produced by FastQC (version 0.11.2)