FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b42.3520000003f747.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b42.3520000003f747.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168344
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA35482.107589222069097No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAA24551.458323432970584No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC23691.4072375611842418No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG21961.3044717958466No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATGAGA16360.9718196074704177No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA15420.9159815615644157No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA14030.8334125362353277No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA12940.7686641638549636No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTATCATAATGGATACTTTTATT11920.7080739438293019No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT11890.7062918785344295No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT11780.6997576391198973No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGT11660.6926293779404078No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG9520.5655087202395096No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT9450.5613505678848073No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTATT9330.5542223067053177No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAATCAACA8110.48175165138050663No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT7720.4585848025471653No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTGT7210.42828969253433447No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG6570.3902722995770565No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTAGCCAAAATTGAA6210.36888751603858766No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGTTTCTTTTATT5950.35344295014969346No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGT5590.33205816661122467No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT5340.3172076224872879No Hit
GGGGGGAGCAGAAGCGGAGCCATGGATGAACTCCACAACGAAATACTAGA5200.30889131777788337No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA4900.29107066482915933No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTAGCCAAAATTGAA4740.2815663165898399No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG3960.23523261892315736No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3600.2138478353846885No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA3190.1894929430214323No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC2600.15444565888894168No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATGAGA2560.15206957182911182No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT2150.12771467946585563No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAA2040.12118044005132349No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTATCATAATGGATACTTTTATT2030.120586418286366No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTG2020.11999239652140854No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTGT1760.10454783063251437No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT19800.0291.275241
CGGGTTA20100.0286.927862
GGGGGGA18650.0279.973181
GTTATTA21000.0276.73815
GGGTTAT21900.0265.36533
TTATTAG22350.0258.702456
ATTAGTA22450.0257.55018
TATTAGT22500.0256.977787
TTAGTAG23150.0249.762429
GGAGCAG22000.0234.659095
GGTTATT24850.0233.863174
GGGGGAG22350.0233.624162
CAGAAGC22250.0231.359549
GGGGAGC22500.0230.755553
GAGCAGA22450.0229.955446
GGGAGCA24350.0213.223824
GCAGAAG26000.0197.99048
AGCAGAA26600.0192.969927
CCCGGTT508.757685E-488.5295
TATTAGG550.001278748480.4545447
Produced by FastQC (version 0.11.2)