FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b41.3520000003f73a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b41.3520000003f73a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248450
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC77543.1209498893137453No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA76063.0613805594687062No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA58452.3525860334071242No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT50632.0378345743610384No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT50242.0221372509559266Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA50182.019722278124371No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA32231.2972429060173074No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT23710.9543167639364056No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG22690.9132622257999597No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA21440.8629502918092171No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC15360.6182330448782452No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT15080.6069631716643188No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC9890.39806802173475553No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG9360.37673576172268064No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT7940.319581404709197No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT7770.31273898168645603No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7100.285771785067418No Hit
CCGGGTTATTAGTAGAAACAACAGCTACCCTTATTGAGCATCTCCTGGAT6590.265244515999195No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT6120.24632722881867578No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT5120.2060776816260817No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4560.183537935198229No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT4450.17911048500704368No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4390.176695512175488No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT4240.1706580800965989No Hit
CCGGGTTATTAGTAGAAACACGAGCGGCGGGGCAGAGAGCAGTGCCACAA4070.16381565707385792No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT4030.16220567518615414No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAA4020.16180317971422822No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTTGTGAACTTCAAGCACCAGTAAA3850.1549607566914872No Hit
CCGGGTTATTAGTAGTAACAAGAGCAAAACTCCATCTCAAAAAAACAAAA3750.15093580197222783No Hit
CCGGGTTATTAGTAGAAACACGTGCGGCGGGGCAGAGAGCAGTGCCACAA3610.14530086536526463No Hit
CCGGGTTATTAGTAGAAACACGAGCAGTATTTGCAGCATAGAGGCATTTA3450.13886093781444958No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT3450.13886093781444958No Hit
GGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAATA3220.12960354196015295No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT3160.1271885691285973No Hit
GGGGGGAGCAGAAGCGGTGCTGTAGGAAGAGCATGGGCTTTAAAATCCGC2840.11430871402696721No Hit
GGGGGGAGCAGAAGCGGAGCGGGGTGTGTGATGTGGGGGTTCTGAGGCCG2740.11028375930770778No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA2660.10706379553230028No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA2630.10585630911652244No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTGCTTCTGCTCCCCCCCCGGGTTA2580.10384383175689274No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT2520.10142885892533708No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT38700.0284.70931
CGGGTTA39450.0279.296572
GGGGGGA33100.0272.719021
GGGTTAT41950.0262.300353
GGTTATT42600.0258.298134
ATTAGTA43000.0255.895348
TATTAGT43250.0254.416177
TTAGTAG44050.0249.795689
GTTATTA44850.0245.668885
GGGGAGC36500.0245.294533
TTATTAG45000.0245.177766
GGAGCAG38500.0231.019475
GAGCAGA39050.0227.765696
CAGAAGC40700.0219.981589
GGGGGAG41550.0215.836322
GCTCGAT304.6629248E-7196.66667295
GGGAGCA48150.0185.944964
GCAGAAG48850.0182.374628
AGCAGAA54600.0163.438647
GCTCGAC752.7212081E-9117.99999295
Produced by FastQC (version 0.11.2)