FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b4.3520000003f48d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b4.3520000003f48d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189101
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAATTCAG26661.4098286101078261No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGC19671.0401848747494726No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTCGTAA19531.03278142368364No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATAAGA19081.008984616686321No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA18340.9698520896240632No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA16230.8582715057033015No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTAACAAGAGC16190.8561562339702063No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGAAACAACAGC14790.7821217233118809No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT13760.7276534761846843No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT11400.6028524439320786No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGAAACAACAGC10660.5637199168698209No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGAAACAACGCA10120.5351637484730382No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT10050.5314620229401219No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCGGGTTATTAGTAGAAACAAC8800.46535978128090283No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC8090.4278137080184663No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGGGGGGAGCAGAAGCAGAGGAT7910.41829498521953873No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGAAACACGAGC7820.41353562382007497No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGAAACAACGCA7690.4066609906875162No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACAAC7310.38656590922311357No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCCAAAATTGAA6600.3490198359606771No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGAGC6240.329982390362822No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA6140.32469421103008445No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTGTTCATTG6060.32046366756389444No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGTAACAAG5850.3093584909651456No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT5640.2982533143663968No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT5630.29772449643312304No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT5480.2897922274340168No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGAAACACGTGC5440.2876769557009217No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCGGGTTATTAGTAGTAACAAG5350.2829175943014579No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGAAACACGTGC5190.2744565073690779No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT4930.2607072411039603No Hit
GGGGGGAGCAGAAGCAGAGGATTGGGGGGAGCAGAAGCACGCTCTTGTTA4750.25118851830503275No Hit
GGGGGGAGCAGAAGCAGAGGATTGGGGGGAGCAGAAGCAGAGGATTGGGG4750.25118851830503275No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATAAGA4740.250659700371759No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAATTCAG4730.25013088243848525No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATGCCTTTATT4610.2437850672392002No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGGGGGGAGCAGAAGCACGCTCT4350.23003580097408263No Hit
CCGGGTTATTAGTAGAAACAACGCACTTCCGGGTTATTAGTAGAAACACG4110.21734417057551256No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT3970.20994071950968No Hit
CCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTAGTAGTAACAAGAGC3790.20042199671075245No Hit
GGGGGGAGCAGAAGCAGAGGATTGGGGGGAGCAGAAGCGGTGCGTTGTTT3650.1930185456449199No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTGGGGGGAGCAGAAGCAGAGGAT3380.17874046144652858No Hit
CCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTAGTAGAAACAACAGC3340.17662518971343358No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTCCGGGTTATTAGTAGAAACACG3300.17450991798033855No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTCGTAA3160.167106466914506No Hit
CCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGTAACAAGAGC3140.1660488310479585No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT3130.16552001311468473No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGGGGGGAGCAGAAGCAGGCACT3050.16128946964849472No Hit
CCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACAACGCA2910.15388601858266218No Hit
CCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACAACAGC2850.15071311098301965No Hit
CCGGGTTATTAGTAGAAACAACAGCCCGGGTTATTAGTAGTAACAAGAGG2780.1470113854501034No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGTGC2770.14648256751682964No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTGTTCATTG2770.14648256751682964No Hit
GGGGGGAGCAGAAGCGGTGCGTTGTTTCTACTAATAACCCGGGCTCTTGT2610.13802148058444957No Hit
GGGGGGAGCAGAAGCGGTGCGTTGTTTCTACTAATAACCCGGGCTGTTGT2540.13431975505153332No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTGTTCATTG2480.13114684745189079No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA2470.13061802951861703No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGGGGGGAGCAGAAGCACGCACT2460.13008921158534328No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCCAAAATTGAA2320.12268576051951074No Hit
CCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTAGTAGAAACAACGCA2310.122156942586237No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATGTTATTAGTAGTAACA2200.11633994532022571No Hit
CCGGGTTATTAGTAGTAACAAGAGCGTGCTTCTGCTCCCCCCAATCCTCT2160.11422467358713068No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAGGCAAATAGGCCAA2140.11316703772058319No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGG2100.11105176598748816No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGGGGGGAGCAGAAGCAGGCTCT2010.10629240458802439No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAAA1960.10364831492165562No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTC1930.10206186112183435No Hit
CCGGGTTATTAGTAGTAACAAGAGCGTGCTTCTGCTCCCCCCAAGTGCGT1910.10100422525528686No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA55150.0125.702631
CCGGGTT106450.0123.875051
CGGGTTA107700.0122.300372
GGGTTAT111550.0118.079333
CGGGTAT505.9256054E-6118.02
TTATTAG112700.0116.8744356
GGTTATT113750.0116.0549554
GTTATTA114050.0115.361685
TATTAGT115350.0115.084527
ATTAGTA116450.0113.3641058
GGGGAGC61400.0112.907173
TTAGTAG118400.0111.497049
AGGTCGT403.6073115E-4110.62499295
GGGAGCA63100.0109.631544
GGAGCAG63600.0109.233485
GGGGGAG64600.0107.77092
GAGCAGA64650.0107.45946
CAGAAGC65950.0104.67029
AGCAGAA66400.0104.182997
GCAGAAG66750.0103.636718
Produced by FastQC (version 0.11.2)