FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b35.3520000003f703.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b35.3520000003f703.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175915
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA28891.6422704146889122No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG27831.582014040872012No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC18321.0414120455901998No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT16130.9169201034590571No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA15390.8748543330585794No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC14580.8288093681607595No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT13370.7600261489924112No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA11670.6633885683426655No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT11420.6491771594235852No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT10540.5991530000284229No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT10510.5974476309581332No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA7840.4456697837023562No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT6770.38484495352869286No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT6540.371770457323139No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAACATAAATCCGTATTTTCT6100.3467583776255578No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5850.33254696870647754No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG5830.33141005599295115No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA5790.3291362305658983No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG5600.3183355597873973No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA5300.30128186908450105No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4830.2745644203166302No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAACATAAATCCGTATTTTCT4030.22908791177557344No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3860.21942415371059887No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3820.21715032828354602No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT3820.21715032828354602No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA3800.2160134155700196No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3600.20464428843475543No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3410.19384361765625446No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT3000.17053690702896285No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAACAAGA2600.14779865275843446No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA2470.14040872012051275No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT1990.11312281499587869No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG1930.10971207685529943No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT1860.10573288235795697Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT15900.0294.072331
CGGGTTA16250.0287.73852
GGGTTAT18150.0257.61713
GGGGGGA16600.0255.90361
TATTAGT18650.0250.710457
ATTAGTA18700.0250.04018
TTATTAG18750.0248.586656
GGAGCAG17500.0242.742865
GGGGAGC17600.0242.201693
GAGCAGA17600.0241.363636
CAGAAGC17900.0238.966499
GGGAGCA18850.0226.140584
GTTATTA20700.0225.881655
TTAGTAG20650.0225.71439
GGTTATT21550.0216.972154
CGGCAGG150.0028476538196.66667295
GGGGGAG22650.0188.20092
GCAGAAG23000.0185.978268
AGCAGAA26450.0161.162577
CGTGGTT200.006732879147.50002295
Produced by FastQC (version 0.11.2)