FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b34.3520000003f6f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b34.3520000003f6f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164347
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA26521.6136589046347058No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG23371.4219912745593164No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA19221.1694767777933275No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC14490.8816710983467906No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA14370.8743694743439187No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT14200.8640255070065167No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCA14090.8573323516705508No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA12810.779448362306583No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT11950.7271200569526672No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT10770.6553207542577595No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT10060.6121194789074336No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT9200.5597911735535178No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG8060.49042574552623414No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT7390.4496583448435323No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA7090.43140428483635235No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT6250.38029291681624855No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT6120.37238282414647056No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA5610.34135092213426466No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA5560.3383085787997347No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5400.32857308012923875No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCA5000.30423433345299883No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT4770.29023955411416086No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG4700.2859802734458189No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3810.23182656209118513No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT3780.23000115609046712No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT3640.22148259475378318No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3560.21661484541853518No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA3550.21600637675162918No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT3380.2056624094142272No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCA3130.19045069274157728No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG2820.17158816406749133No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT2030.12351913938191753Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAA1800.10952436004307958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT17300.0292.44221
CGGGTTA17800.0285.056152
GGGTTAT19350.0262.222233
GGGGGGA16000.0258.1251
TATTAGT20100.0251.703987
GGGGAGC16600.0249.683753
TTATTAG20700.0243.695656
ATTAGTA20850.0243.357328
GTTATTA21150.0237.813235
TTAGTAG21300.0237.523479
GGAGCAG17600.0233.821015
GGTTATT21750.0232.60924
CAGAAGC18000.0229.444439
GAGCAGA18350.0224.264316
GGGAGCA19000.0217.368444
GCAGAAG21050.0196.199528
GGGGGAG21500.0193.46512
AGCAGAA23550.0174.745247
GTACGCC200.0067324797147.49998295
CCACGTT652.1807558E-590.76923295
Produced by FastQC (version 0.11.2)