FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b33.3520000003f6e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b33.3520000003f6e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139701
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA28212.019312674927166No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA22981.644941696909829No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA22841.6349202940565923No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC20011.4323447935233107No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA18111.2963400405151002No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG17161.2283376640109949No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT12120.867567161294479No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC11020.7888275674476203No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT10570.7566159154193599No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT9690.693624240341873No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT9250.6621284028031295No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7850.5619143742707641No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA7050.5046492151094122No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT6900.4939119977666588No Hit
CCGGGTTATTAGTAGAAACACGAGCATCTATTGGCACTTGAGAGAAAACT5950.42590962126255355No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT5630.4030035575980129No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG5550.3972770416818777No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT5250.3758026069963708No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG4940.35361235782134703No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA4740.3392960680310091No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4620.3307062941568063No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT4330.30994767396081635No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA4210.30135790008661356No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA4180.29921045661806284No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT4090.29276812621241083No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT4080.29205231172289386No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4020.2877574247857925No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT4010.2870416102962756No Hit
CCGGGTTATTAGTAGAAACACGTGCATCTATTGGCACTTGAGAGAAAACT3680.26341973214221803No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3220.23049226562444078No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2410.17251129197357215No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT2360.16893221952598764No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG1970.14101545443482869No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT1930.13815219647676108No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG1830.1309940515815921No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA1580.11309868934366969No Hit
CCGGGTTATTAGTAGTAACAAGAGCAGTACTCAAACAAATGAGAGACCAA1460.10450891546946693No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT18350.0290.980931
CGGGTTA18600.0287.862882
GGGGGGA16400.0270.716461
ATTAGTA20250.0265.86428
TATTAGT20200.0265.792087
GGGTTAT20300.0263.756163
TTAGTAG20650.0260.71439
TTATTAG21050.0252.957266
GGTTATT21200.0251.86324
GTTATTA21600.0247.199085
GGGGAGC18300.0242.60933
GGAGCAG18750.0236.000025
CAGAAGC19150.0231.070519
GAGCAGA19450.0228.264796
GGGGGAG21550.0206.705322
GGGAGCA21550.0206.020874
GCAGAAG22300.0199.091928
AGCAGAA28100.0157.473317
CGGGCTA200.006731406147.52
CTATCTT754.43273E-578.66667295
Produced by FastQC (version 0.11.2)