FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b32.3520000003f6db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b32.3520000003f6db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132458
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA26812.0240378082109043No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA26642.0112035513143787No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA23811.7975509218016277No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA23211.7522535445197724No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC21001.585408204864938No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT16061.2124597985776624No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT11450.8644249497954068No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG11360.8576303432031286No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTGTTTGCTGACAT9900.7474067251506138No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT9670.7300427305259025No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT8240.6220839813374806No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7790.588110948376089No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA7700.5813163417838108No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC7020.529979314197708No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA6830.5156351447251204No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT5700.43032508417762616No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT5490.4144710021289767No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4810.36313397454287394No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT4810.36313397454287394No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT4370.32991589786951336No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAACAAAGAAAACAAAAAAGAAT3200.24158601216989536No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT3170.23932114330580256No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG2970.22422201754518414No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT2780.2098778480725966No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA2780.2098778480725966No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2750.2076129792085038No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG2570.19402376602394722No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA2430.1834543779915143No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT2390.1804345528393906No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2250.16986516480695768No Hit
GGGGGGAGCAGAAGCAGAGGATTCTGATTCTTGAATACCTTTTTTTCCTT2020.15250117018224646No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA1880.14193178214981353No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT1800.13589213184556614No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1600.12079300608494768No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAA1470.11097857434054569No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT1440.10871370547645293Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAT1390.1049389240362983No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG1340.10116414259614369No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG1330.10040918630811276No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGG108.456588E-4295.0295
CCGGGTT15150.0288.18481
CGGGTTA15500.0283.580632
GGGGGGA16950.0270.634221
GGGGAGC17750.0258.436653
TATTAGT17500.0254.542857
ATTAGTA17550.0253.817668
TTATTAG18000.0246.652796
TTAGTAG18250.0244.08229
GTTATTA18500.0239.986485
GGAGCAG19150.0239.543085
GGGTTAT18550.0238.544483
GGTTATT18750.0236.04
GGGGGAG20100.0227.487582
CAGAAGC20400.0227.034329
GAGCAGA20700.0221.606286
GGGAGCA21950.0208.986314
CCGATAG150.002846863196.66667295
TCGGGTT150.002846863196.666671
CGGGCTA150.002846863196.666672
Produced by FastQC (version 0.11.2)