FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b31.3520000003f6ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b31.3520000003f6ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144153
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT31322.172691515265031No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT24591.7058264482875833No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA20591.4283434961464554No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT19421.3471797326451758No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCACAATGGATACTTTTATT18831.3062509972043592No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA18561.2875208979348332No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA16741.16126615471062No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC15801.0960576609574548No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT14961.0377862410078182No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA14210.9857581874813566No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA14200.9850644801010038No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT13830.9593973070279495No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT13220.9170811568264275No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG12010.8331425638037362No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT11820.8199621235770328No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG11350.7873578767004502No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA10630.7374109453150473No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT9200.638210789924594No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC8160.5660652223679008No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT7780.5397043419144937No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7580.5258301943074372No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT7500.5202805352646147No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG7360.5105686319396752No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT6140.42593633153663124No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG5270.36558378944593595No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5190.3600341304031134No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA5090.3530970565995852No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTTT4670.3239613466247667No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA4030.27956407428218627No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAACAAGA3590.24904094954666225No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG3290.22822972813607761No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCACAATGGATACTTTTATT3230.22406748385396075No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGC2760.1914632369773782No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA2650.1838324557934972No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATT2560.1775890893703218No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2510.1741205524685577No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCTA2430.16857089342573517No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTAT2380.16510235652397107No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTATTCATTG2030.14082259821162238No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT1980.13735406130985828No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG1950.13527293916879982No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT1870.12972328012597728No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1860.12902957274562443No Hit
GGGGGAGCAGAAGCGGTGCGTTTGATTTGTCACAATGGATACTTTTATTA1700.11793025465997933No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATTC1530.1061372291939814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT21850.0290.27461
CGGGTTA21900.0289.611882
GGGGGGA21050.0282.387181
TATTAGT22800.0274.945167
ATTAGTA23150.0272.062658
TTAGTAG23750.0265.189489
TTATTAG23650.0264.439736
GTTATTA24100.0261.95025
GGGGAGC24100.0248.485473
GGGTTAT26050.0243.47413
GGGGGAG24900.0241.094392
GGTTATT26400.0239.128784
GGAGCAG24950.0237.064135
CAGAAGC25550.0230.919779
GAGCAGA25900.0228.368736
GGGAGCA26350.0226.707784
GCAGAAG29100.0203.76298
AGCAGAA31150.0190.353137
TCTCCGC508.755115E-488.5295
GGGTACC600.001805666473.75295
Produced by FastQC (version 0.11.2)