FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b3.3520000003f470.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b3.3520000003f470.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154380
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTCGTAA27841.8033424018655269No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAATTCAG26821.737271667314419No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT17781.1517035885477394No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA16341.0584272574167637No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC13560.8783521181500195No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA13220.856328539966317No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT13070.8466122554735069No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT12610.8168156496955564No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATAAGA11890.7701774841300687No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATGCCTTTATT9520.6166601891436714No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT9140.5920456017618863No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA8480.5492939499935224No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT8230.5331001425055059No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCCAAAATTGAA8200.531156885606944No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT8170.5292136287083818No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTGTTCATTG7840.5078378028242001No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT6920.44824459126829896No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT6730.4359372975774064No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT5160.33424018655266224No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAATTCAG4930.319341883663687No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTGAAGAAAATGC4800.3109211037699184No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTCGTAA4720.3057390853737531No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTGTTCATTG4290.27788573649436454No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCCAAAATTGAA3820.24744137841689337No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAGGCAAATAGGCCAA3580.23189532322839745No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTGTTCATTG3430.22217903873558753No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA3060.19821220365332298No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATAAGA2660.17230211167249646No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT2600.16841559787537247No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTGAAGAAAATGCTGGGC2480.1606425702811245No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCTC2380.15416504728591784No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2190.14185775359502525No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT2110.13667573519885998Illumina Single End PCR Primer 1 (97% over 34bp)
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTTT2090.13538023059981863No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAAA1850.11983417541132271No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAA1770.11465215701515741No Hit
CCGGGTTATTAGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGT1550.1004016064257028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT16750.0292.358221
CGGGTTA17100.0285.51172
GGGGGGA14200.0278.380251
GGGGAGC15450.0252.993553
TTATTAG19650.0248.460566
TATTAGT19850.0245.957177
GGAGCAG15900.0245.833335
CAGAAGC15950.0244.137929
ATTAGTA20050.0242.768078
GTTATTA20150.0242.295275
GGGTTAT20250.0241.827153
GGGAGCA16500.0235.999984
GAGCAGA16800.0232.66376
GGTTATT21550.0227.238974
TTAGTAG22550.0217.161889
GCAGAAG18200.0215.576928
AGCAGAA18650.0208.793567
GGGGGAG19900.0199.384412
GCGAAGG150.0028473176196.66666295
CGCGATT150.0028473176196.66666295
Produced by FastQC (version 0.11.2)