FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b29.3520000003f6b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b29.3520000003f6b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131512
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT30762.338950057789403No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT25881.9678812579840623No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA20801.5816047204817811No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA20171.5337003467364194No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC19701.49796216314861No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT18481.4051949631972749No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT16701.269846097694507No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT16541.257679907536955No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT15121.1497049698886794No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA12980.9869821765314192No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA11680.8881318815013078No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTGTTCATTG8820.6706612324350629No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA8720.6630573635865928No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA8480.6448080783502647No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT8460.6432873045805706No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT7030.5345519800474482No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA6930.5269481111989781No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG6450.4904495407263215No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTTT5500.41821278666585554No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTGTTCATTG5280.40148427519922136No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTGTTCATTG4940.37563112111442304No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT4920.374110347344729No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4640.3528195145690127No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA4640.3528195145690127No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT4420.3360910031023785No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3070.2334387736480321No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATT2810.21366871464200987No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT2720.2068252326783868No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG2710.20606484579353976No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTAT2630.19998175071476368No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT2460.1870551736723645No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2420.18401362613297645No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCTA2210.16804550155118925No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT2190.1665247277814952Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAGAAGCAGAGGATTTTCTTATGAACTTCAAGCACCAGTAAA2070.1574000851633311No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATTC1990.15131699008455501No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC1650.12546383599975666No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT1620.12318267534521564No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1590.12090151469067462No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA1490.1132976458422045No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCACTCAATTGTGTTCATTG1440.10949571141796947No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTCTCT1400.10645416387858142No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTGTTCATTG1380.1049333901088874No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAA1330.10113145568465234No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT18850.0290.305051
CGGGTTA19150.0285.757172
GGGGGGA16800.0274.806551
GTTATTA20350.0269.631445
TATTAGT20550.0266.289557
ATTAGTA20600.0264.92728
TTAGTAG20850.0261.75069
TTATTAG21550.0254.617166
GGGGAGC18850.0244.137943
GGGGGAG19400.0237.97682
GGGTTAT24050.0228.149693
GGAGCAG20450.0227.20055
GGTTATT24450.0224.417184
GAGCAGA20700.0222.318856
CAGAAGC21350.0214.859489
GGGAGCA21650.0213.92614
GCAGAAG24500.0187.836728
AGCAGAA24800.0184.969777
GTAGCTT351.0032236E-6168.57143295
TTGGACC508.753394E-488.56
Produced by FastQC (version 0.11.2)