FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b28.3520000003f6a5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b28.3520000003f6a5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101535
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA36333.578076525336091No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA33333.28261190722411No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC32293.1801841729452898No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT17641.737331954498449No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA12021.183828236568671No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT11721.1542817747574727No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG9870.9720785935884179No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT8920.8785147978529572No Hit
GGGGGGAGCAGAAGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT7430.7317673708573398No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC7140.703205791106515No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT6270.6175210518540405No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC5330.5249421381789531No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA5320.5239572561185798No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT4440.437287634805732No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT4190.41266558329640024No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA3790.37327030088146945No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG3680.36243659821736346No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT2710.26690303836115625No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC2450.24129610479145122No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT2380.23440193036883833No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2380.23440193036883833No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2070.20387058649726694No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA1830.18023341704830848No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAT1800.17727877086718866No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC1760.17333924262569558No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT1580.1556113655389767No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT1480.145762544935244No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT1370.13492884227113805No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT1330.13098931402964495Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG1300.12803466784852513No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT1290.12704978578815188No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA1210.11917072930516572No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT1080.1063672625203132No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA1020.10045797015807358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT13650.0293.91941
GGGGGGA12200.0282.909851
CGGGTTA14250.0280.50882
GGGTTAT14550.0272.697573
GGTTATT14800.0269.087834
TATTAGT14950.0264.41477
ATTAGTA15200.0261.036168
TTAGTAG15300.0259.330059
GGGGAGC13400.0255.373143
GGAGCAG13650.0247.454215
GTTATTA16150.0245.68115
CAGAAGC14150.0238.710259
GAGCAGA14300.0237.237766
TTATTAG16950.0233.215356
GGGGGAG15650.0220.54312
GGGAGCA16200.0211.234564
GCAGAAG18250.0186.698648
AGCAGAA20000.0170.362527
CGCGAAA301.1460172E-4147.49998295
GACGTGG352.1174035E-4126.428566295
Produced by FastQC (version 0.11.2)