FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b24.3520000003f63b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b24.3520000003f63b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159633
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA29321.8367129603528092No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG20841.305494477958818No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAAGAAAAA20301.271666885919578No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC19721.2353335463218758No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA15660.9810001691379602No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT15080.9446668295402579No Hit
CCGGGTTATTAGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGT13860.8682415290071601No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC13360.8369196845263823No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAA11970.7498449568698201No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA11650.7297989764021223No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT11070.6934656368044201No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT9150.5731897539982335No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT9150.5731897539982335No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT8170.511798938815909No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAAT7180.44978168674396896No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC6340.3971609880162623No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA5690.3564425901912512No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT4810.30131614390508227No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT4620.2894138430023867No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG4310.2699942994243045No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT4090.25621268785276224No Hit
GGGGGGAGCAGAAGCGGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC3800.23804601805391115No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3740.23428739671621782No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG3590.22489084337198448No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA3510.21987934825506003No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA3400.21298854246928892No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3310.2073506104627489No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT2600.16287359130004447No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2570.1609942806311978No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2300.1440804846115778No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT2120.1328046205984978No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT2110.13217818370888224No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG2030.1271666885919578No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA1920.12027588280618669No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA1880.11777013524772446No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG1640.10273564989695112No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG1600.10022990233848891No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT18850.0291.870031
CGGGTTA19200.0285.013032
GGGTTAT20950.0261.90933
TATTAGT21800.0253.050467
GGTTATT21850.0250.446234
ATTAGTA22050.0249.512478
GGGGAGC17300.0246.401733
TTATTAG22450.0244.409796
GGGGGGA17750.0240.985931
GTTATTA23250.0236.05
TTAGTAG24550.0224.103879
CAGAAGC19300.0221.632139
GGAGCAG19750.0215.835455
GAGCAGA20550.0208.150866
GGGAGCA20700.0204.504844
GCAGAAG23100.0185.173168
GGGGGAG23150.0184.773212
AGCAGAA25150.0170.6667
GCTCGAT351.0038893E-6168.57144295
CCGCGTT200.006732299147.5295
Produced by FastQC (version 0.11.2)