FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b2.3520000003f463.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b2.3520000003f463.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences291740
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC84262.8881881127030917No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA82082.8134640433262494No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTCGTAA77432.6540755467196817No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATAAGA71372.4463563446904777No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA66792.28936724480702No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAATTCAG41091.4084458764653458No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT35721.2243778707067938Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT35251.2082676355659148No Hit
CCGGGTTATTAGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGT32411.110920682799753No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA30311.0389387811064645No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAA29581.0139165009940359No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAAT28180.9659285665318434No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT25420.8713237814492356No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTGAAGAAAATGC24810.8504147528621376No Hit
GGGGGGAGCAGAAGCGGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC14770.5062727085761294No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTCGTAA14650.5021594570507987No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAATAAGA11000.3770480564886543No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAATTCAG9830.3369438541166792No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAGGCAAATAGGCCAA7660.26256255570028103No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAA6900.2365119627065195No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT6630.22725714677452527No Hit
GGGGGGAGCAGAAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC5740.1967505312949887No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAT5240.17961198327277714No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT5100.17481318982655789No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAGGCAAATAGGCCAAA5060.17344210598478096No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATGCCTTTATT5010.1717282511825598No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT4930.16898608349900596No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT4500.154246932199904No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT4080.13985055186124631No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAA3660.1254541715225886No Hit
CCGGGTTATTAGTAGAAACAACAGCTCCCTGCCCCATACGTGGATGACTC3200.10968670734215398No Hit
GGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATA3170.10865839446082128No Hit
GGGGGGAGCAGAAGCAGGCACTTGGACACATTCTGTATTCATCACTGCCA3160.10831562350037705No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT3120.10694453965860012No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTGTTCATTG3010.10317405909371358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT56050.0289.736821
CGGGTTA57100.0283.6342
GGGGGGA39650.0277.143771
GGGTTAT58800.0275.182833
GGTTATT59900.0269.63694
TATTAGT61050.0267.94027
ATTAGTA61400.0266.412848
TTATTAG64150.0252.69296
TTAGTAG65050.0252.14459
GGGGAGC43550.0251.986223
GTTATTA65350.0247.375665
CAGAAGC47000.0234.430859
GGAGCAG48750.0225.410265
GAGCAGA49750.0221.472356
GCAGAAG51900.0212.58198
GGGGGAG52000.0211.038452
AGCAGAA52900.0207.726847
GGGAGCA54150.0202.386894
CGGGCTA603.6379788E-12172.083342
CGGTTTA655.456968E-12158.846162
Produced by FastQC (version 0.11.2)