FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b19_pb.3520000003f5f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b19_pb.3520000003f5f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263540
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT133505.06564468391895No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCT115274.373909084010017No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT110904.2080898535326705No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA106134.027092661455566No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG91793.4829627381042725No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG74682.83372543067466No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT57722.190179858844957Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA43051.6335281171738634No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCT41791.5857175381346285No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT28451.0795325187827274No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT25850.980875768384306No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATT24250.9201639219852774No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTA23190.879942323745921No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATTC20500.7778705319875541No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT19620.7444790164680883No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT18360.6966684374288533No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTAT16420.6230553236700311No Hit
GGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATT13520.5130151020717917No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT13110.49745769143204066No Hit
GGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTG13100.49707824239204673No Hit
CCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTAGTAGAAACACGAGC11480.4356074979130303No Hit
CCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGTGC11230.42612127191318205No Hit
CCGGGTTATTAGTAGAAACACGTGCCCGGGTTATTAGTAGAAACACGTGC11140.4227062305532367No Hit
GAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATTCA10660.40449267663352817No Hit
CCGGGTTATTAGTAGAAACACGAGCCCGGGTTATTAGTAGAAACACGAGC8210.31152766183501557No Hit
GGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGT7460.2830689838354709No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCTA7270.27585945207558626No Hit
GGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAAT7140.2709266145556652No Hit
GGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCTATT6420.2436062836761023No Hit
GGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCTAT4960.1882067238369887No Hit
GCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATTCATA4710.17872049783714047No Hit
GGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCTATTC4570.17340821127722547No Hit
AGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATTCAT4530.17189041511724976No Hit
GGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAATT4130.1567124535174926No Hit
CGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCTA3990.1514001669575776No Hit
GAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTGTT3520.13356606207786295No Hit
CGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCTA3350.12711542839796613No Hit
GGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAATTG3280.12445928511800866No Hit
GGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATTA3140.11914699855809364No Hit
CGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTGA2770.1051073840783183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA45950.0264.50491
CCGGGTT65950.0257.202421
CGGGTTA69050.0246.509782
GTTATTA69550.0243.889285
GGGTTAT70250.0242.50893
TTATTAG70700.0239.922236
GGGGGAG51150.0237.038132
ATTAGTA72050.0235.222058
GGTTATT72350.0235.062214
TATTAGT72500.0233.965537
TTAGTAG75300.0225.26569
GGGGAGC56350.0214.902393
GGGAGCA60550.0200.483064
ATTTGCT2150.0192.09302295
GGAGCAG65400.0186.066515
GAGCAGA67500.0180.277796
AGCAGAA68050.0178.820727
GCAGAAG69150.0177.042668
CGGTTAT255.5516673E-5177.02
CAGAAGC69400.0175.767299
Produced by FastQC (version 0.11.2)