FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b19.3520000003f5e9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b19.3520000003f5e9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199296
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA53032.660866249197174No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA45832.299594572896596No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA34581.7351075786769428No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC31411.5760476878612717No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT31171.5640052986512523No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCT31091.5599911689145793No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCT29461.478203275529865No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA24691.2388607899807322No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA23921.2002247912652537No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG22431.1254616249197174No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA21071.0572214193962748No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT19290.9679070327552987No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT19060.9563664097623634No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG16720.8389531149646757No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA14840.7446210661528581No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT13480.6763808606294155No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT13430.6738720295439948No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG13070.655808445728966No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC12010.6026212267180475No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT11350.5695046563904946No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT11100.5569605009633912No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT9650.48420439948619143No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC8030.4029182723185613No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA7630.38284762363519587No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG6680.33517983301220294No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTACTTTCT6530.3276533397559409No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT5970.2995544315992293No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT5790.2905226396917148No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG5370.2694484585741811No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5110.2564025369299936No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTA4580.22980892742453435No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4560.2288053949903661No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTATTCATTG4520.22679833012202955No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT4350.21826830443159922No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA4260.213752408477842No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT3050.15303869621066152No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCTA3020.15153339755940912No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTAT2700.13547687861271676No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATT2650.1329680475272961No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAA2640.13246628131021193No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG2520.12644508670520233No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT2510.12594332048811818No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2350.117915061014772No Hit
AGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAAAATAGACACTA2290.11490446371226717No Hit
GCAGAGGATTATGATGACCTCGATTATTAAAGCAACAAAATAGACACTAT2220.11139210019267823No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAT2220.11139210019267823No Hit
GGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAAT2020.1013567758509955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT33200.0290.557251
CGGGTTA33350.0288.365842
GGGTTAT33800.0284.526643
TATTAGT34650.0280.1017
GGTTATT34500.0279.181154
ATTAGTA34900.0277.249278
GGGGGGA33500.0274.305971
TTAGTAG35650.0271.83039
GTTATTA36450.0263.840885
TTATTAG36750.0262.088446
GGGGAGC37600.0244.787253
GGAGCAG39950.0230.018775
GGGGGAG41100.0224.659382
GAGCAGA41100.0223.94166
CAGAAGC41050.0223.136439
GGGAGCA43250.0212.46824
GCAGAAG45550.0201.09228
CGCCTCT150.002847937196.66666295
AGCAGAA50200.0183.052787
GGGCTAG200.0067335465147.5295
Produced by FastQC (version 0.11.2)