FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b18.3520000003f5dc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b18.3520000003f5dc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186028
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA37322.0061496118863826No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA34421.8502591007805276No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA25411.3659234093792332No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAGCAATCAAGTTCAG23651.2713139957425763No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCA15460.8310576902401788No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT13280.7138710301675016No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT13240.7117208162212141No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC12800.6880684628120498No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT12430.668178983808889No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA12330.6628034489431699No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT11440.6149611886382694No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT10600.5698066957662287No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTTTTATATT10270.5520674307093556No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT8980.48272303094157865No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA7590.4080030963080826No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA7000.37628744060033975No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG6960.3741372266540521No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTTTTATATT6430.34564689186574066No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT6110.3284451802954394No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT5710.30694304083256285No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT5700.30640548734599093No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAGCAATCAAGTTCAG5070.2725396176919604No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4840.26017588750080634No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA4700.25265013868879954No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG3330.17900531102844733No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCA3210.17255466918958437No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA3190.17147956221644053No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3120.16771668781043714No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCA3060.16449136689100566No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG2760.14836476229384823No Hit
GGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACAA2660.14298922742812908No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT2120.11396133915324576No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT2110.11342378566667384No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT1990.10697314382781087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT21400.0290.86451
CGGGTTA21700.0286.843322
TTATTAG23150.0269.514046
TATTAGT23250.0268.354837
ATTAGTA23550.0263.683658
GTTATTA24600.0253.028465
GGGGGGA19950.0252.857161
GGGTTAT24800.0250.98793
GGGGAGC20500.0246.073183
TTAGTAG25500.0244.676479
GGAGCAG20650.0240.71435
GGGAGCA21350.0236.276354
GAGCAGA21450.0231.048976
GGTTATT27350.0227.586844
CAGAAGC23250.0211.892479
GCAGAAG24550.0202.474558
GGGGGAG25350.0199.575932
AGCAGAA27800.0178.803967
AGGTACT200.0067331884147.5295
TAAGGGC700.003328697363.2142837
Produced by FastQC (version 0.11.2)