FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b17.3520000003f57f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b17.3520000003f57f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258895
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC51661.995403541976477No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA50281.9421000791826803No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA43941.697213155912629No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT39031.507560980320207Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG30501.1780837791382608No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT30311.1707448965796945No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC25020.9664149558701404No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT21470.8292937291179822No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT21120.8157747349311496No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA17320.6689970837598254No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA16950.6547055756194596No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC13990.5403735104965333No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT13160.508314181424902No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT13050.5040653546804689No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA11090.4283589872342069No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT10440.40325228374437516No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT10340.39938971397670875No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG9720.3754417814171769No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA8970.3464725081596786No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC8000.3090055814133143No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG7440.2873751907143823No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC6770.2614959732710172No Hit
CCGGGTTATTAGTAGTAACAAGAGCGAAACTCCATCTCAAAACAAAATAA6330.24450066629328493No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT6220.24025183954885185No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT6110.23600301280441877No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT5940.22943664419938586No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT5570.21514513605902005No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG5440.21012379536105372No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA5340.20626122559338728No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA5270.2035574267560208No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC5060.1954460302439213No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAAT4870.18810714768535505No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT4790.18501709187122192No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG4120.15913787442785685No Hit
CCGGGTTATTAGTAGAAACAACAGCAAGGGAGTTAGAATAAAGACCCAAA4050.15643407559049036No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT4030.15566156163695707Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAGAAGCGGAGCCCAGGGGGAGTCTCCCCAGTCGATGGGGGA3760.1452326232642577No Hit
CCGGGTTATTAGTAGAAACAACAGCCATTATCTCACAGTTTCTATGGGTC3560.13750748372892485No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA3510.13557619884509164No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG3400.13132737210065856No Hit
CCGGGTTATTAGTAGTAACAAGAGCCAGAGACTGACAACCCAGGGACTGG3320.12823731628652543No Hit
CCGGGTTATTAGTAGAAACAACAGCGACAGCCACATGGAATTTGTAAAAT3140.12128469070472586No Hit
GGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAAA2960.11433206512292628No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT32800.0290.503051
CGGGTTA33600.0284.90332
GGGGGGA30100.0267.558171
TATTAGT36850.0256.573947
ATTAGTA36600.0256.31158
GGGTTAT37300.0255.85123
TTATTAG37250.0254.610736
GGGGAGC32800.0246.882613
GTTATTA38950.0244.255465
TTAGTAG38450.0243.595579
GGAGCAG33450.0241.644265
GAGCAGA34150.0236.259166
CAGAAGC34200.0235.913749
GGTTATT40800.0234.26474
GGGAGCA37600.0214.581124
GGGGGAG39150.0206.462332
GCAGAAG42150.0191.417568
AGCAGAA47350.0169.772967
TCGGGTT200.0067347027147.499981
GAGCCGA200.0067347027147.499986
Produced by FastQC (version 0.11.2)