FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b15.3520000003f562.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b15.3520000003f562.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346342
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT63811.8423985540304093No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC60421.7445184239855402No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA56871.642018582788111No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA56711.637398871635551No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT48121.389378129132476Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG47741.3784063151451456No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA45351.3093993798037777No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT40881.1803361994791275No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC40491.169075653544762No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG38951.1246109337013703No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT36351.0495406274722672No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA33610.970428073984674No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT29940.8644634494228248No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA29420.8494493881770043No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT27840.8038297405454723No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG24190.6984425798776932No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTG24100.6958439923543781No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT20180.5826610691166535No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT18120.5231822880274412No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT17840.5150977935104608No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCACTCAATTGTATTCATTG17560.5070132989934804No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT17190.49633021695318497No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC14520.41923878709483686No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC13640.3938303757557559No Hit
CCGGGTTATTAGTAGAAACAACGCACTTGAGAGAAAACTAAAGAAAATGC12070.34849946007125904No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA11960.3453234086538739No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTATTCATTG9220.26621085516628074No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA8660.2500418661323201No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA8530.24628835082086492No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTCT7770.22434472284620405No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAATCATTTGTCT7650.22087993948178392No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT7330.21164051717666352No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT7170.20702080602410336No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT5970.17237297237990196No Hit
GGGGGGAGCAGAAGCAGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGT5600.1616898903396065No Hit
GGGGGGAGCAGAAGCGGTGGAGACGGAGGGTCTGGCCAAATAAGCTGCAG5430.15678144724001133No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTA5210.15042934440524106No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATT5170.14927441661710103No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT5050.14580963325268087Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG5030.14523216935861086No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTGGCTCCCTATTTGTGAGTGGAA4750.1371476748416305No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG4610.13310542758314037No Hit
GGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATTG4510.13021810811279025No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCTATTC4340.12530966501319504No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT4100.11838009828435477No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC4100.11838009828435477No Hit
GGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATT4080.11780263439028474No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTGATTCTTGGTTCTTTATA3670.10596462456184928No Hit
GGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAAT3670.10596462456184928No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGG108.4624777E-4295.0295
CCGGGTT61350.0290.672361
CGGGTTA62500.0284.852022
GGGGGGA46300.0276.20411
TATTAGT67050.0265.082037
ATTAGTA67050.0264.862068
GTTATTA67450.0263.94745
GGGTTAT69850.0255.722983
TTATTAG69700.0255.215216
TTAGTAG69800.0254.426939
GGGGAGC52100.0246.022063
GGTTATT73100.0244.353624
GGGGGAG53800.0238.796452
GGAGCAG56600.0225.680225
GAGCAGA58350.0219.670096
CAGAAGC59000.0216.750029
GGGAGCA63750.0200.64
GCAGAAG71450.0179.394678
CGGGGTA351.0055701E-6168.571432
CCGGGGT401.9550007E-6147.51
Produced by FastQC (version 0.11.2)