FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b14_pb.3520000003f556.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b14_pb.3520000003f556.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494987
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTCT131272.651988840110952No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCT125762.5406727853458775No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA97081.9612636291458159No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTCT96681.9531826088361917No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG89801.8141890595106538No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT72061.4557958087788165Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT70481.4238757785558005No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT62381.2602351172859085No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG60251.2172036841371592No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCT49531.000632339839228No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT41380.8359815510306332No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA34820.7034528179527947No Hit
GGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTA20460.4133441888372826No Hit
GGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATT18050.36465604147179626No Hit
GGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAAT14980.3026342105954298No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTTATT12310.24869340002868762No Hit
GGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTATTC11840.23919820116487908No Hit
GGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTACTTTCTAT10390.20990450254249104No Hit
GGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTACTTTCTA7510.1517211563131961No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT7320.14788267166612457Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATTA7200.1454583655732373No Hit
GGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAAT5310.1072755446102625No Hit
GGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAATT5080.10262895793222851No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT53000.0289.155671
CGGGTTA53550.0286.73672
GTTATTA54850.0279.402925
GGGTTAT58950.0260.220523
TTATTAG59300.0259.430856
GGGGGGA48850.0251.218021
GGGGGAG49650.0247.170182
TATTAGT64300.0239.257397
ATTAGTA64350.0238.613058
GGTTATT69550.0221.196984
GGGAGCA56900.0215.676624
GGAGCAG58050.0211.403965
GGGGAGC58400.0209.884413
GAGCAGA58950.0207.926216
GCAGAAG59600.0206.4018
CAGAAGC59800.0205.217399
AGCAGAA60000.0204.533337
TTAGTAG75300.0203.522589
CCGCAAA609.9416866E-8122.91667295
ATTTGCT2800.0105.35714295
Produced by FastQC (version 0.11.2)