FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b12b.3520000003f53c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b12b.3520000003f53c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154634
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGAAACAACAGCCAACTATGTGTGTGAATGAGTTGGT50553.269009402848015No Hit
CCGGGTTATTAGTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAA49883.2256812861337094No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC28341.8327146681842286No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA27181.757698824320654No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAAT22681.4666890851947179No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTGACAAGA17141.1084237619152322No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA15801.0217675284866201No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA14350.9279977236571517No Hit
GGGGGGAGCAGAAGCGGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC13910.8995434380537268No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGTATTCATTG12280.7941332436592211No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA11040.7139438933222966No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG11030.7132972050131278No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA10290.6654422701346405No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT10060.6505684390237594No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAACCATTTGTTT9810.6344012312945407No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT7870.5089436993158037No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT7210.46626227091066647No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC7000.4526818164181228No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT6920.4475083099447728No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGTATTCATTG6580.42552090743303544No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT6470.4184073360321792No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTGTATTCATTG5430.3511517518786295No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAACCATTTGTTT5380.3479183103327858No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT5120.3311044142943984No Hit
GGGGGGAGCAGAAGCAGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC4590.2968299339084548No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA4460.2884229858892611No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAACCATTTGTTT4360.2819561027975736No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTTGATTCTTGGTTCTTTATA4290.2774292846333924No Hit
GGGGGGAGCAGAAGCGGTGCTGTTTCCTGGGTCAGATAATGGCCTCTGTC4270.2761359080150549No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG4040.26126207690417375No Hit
CCGGGTTATTAGTAGAAACAACAGCGATCCGAAATCCCCACTGCATAGAC3430.22181409004488017No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC3240.209527012170674No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTCTGGGTAACAGTGAACACGGGGA2790.18042603825808037No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC2560.1655522071471992No Hit
CCGGGTTATTAGTAGTAACAAGAGCGAAACTCCATCTCAAAAAAAAAAAA2410.15585188250966797No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTTGATTCTTGGTTCTTTATA2390.1545585058913305No Hit
GGGGGAGCAGAAGCGGAGCTGTTTCCTGGGTCAGATAATGGCCTCTGTCA2340.15132506434548676No Hit
CCGGGTTATTAGTAGAAACAACAGCCAGCGGAAGACAAGTTTAGTTCCAA2320.15003168772714925No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC2160.13968467478044932No Hit
CCGGGTTATTAGTAGTAACAAGAGCGAAACCCCATCTCAAAAAAACAAAA2090.13515785661626809No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT2020.13063103845208687No Hit
CCGGGTTATTAGTAGAAACAACAGCCTTTTTTCATTTTAACCATTTGTTT1970.12739759690624314No Hit
CCGGGTTATTAGTAGAAACACGTGCCTCTGGTTGAGGTCCTTAACTTCAG1930.12481084366956816No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTGTCAGCCTCTCACACTCTGGG1930.12481084366956816No Hit
CCGGGTTATTAGTAGTAACAAGAGCATAGAAAACCAAGCAAATGAGAAAA1910.12351746705123064No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTGACAAGA1900.1228707787420619No Hit
CCGGGTTATTAGTAGAAACAACAGCACTAAGAAGAGAACCTCTTCCTCCA1850.11963733719621816No Hit
CCGGGTTATTAGTAGAAACAACAGCAAGGGAGTTAGAATAAAGACCCAAA1830.11834396057788067No Hit
GGGGGGAGCAGAAGCAGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA1810.11705058395954318No Hit
CCGGGTTATTAGTAGAAACAACAGCTCTCGAGATTGGGAATAGGTAATAA1790.11575720734120568No Hit
CCGGGTTATTAGTAGAAACAACAGCTGCAGGAGGGACTGGGAACACTCCA1760.11381714241369945No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCGTATTTTCT1690.10929032424951822No Hit
CCGGGTTATTAGTAGAAACACGAGCCTCTGGTTGAGGTCCTTAACTTCAG1670.10799694763118073No Hit
CCGGGTTATTAGTAGAAACAACAGCACTGGGTCCATGGGGCTCCGCTTCC1600.10347012946699949No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTCACTCAATTGTATTCATTG1550.10023668792115577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT38750.0293.096741
CGGGTTA39100.0291.227632
TATTAGT40950.0278.79127
GGGGGGA21500.0277.848821
ATTAGTA41600.0275.144238
GTTATTA41550.0274.055365
TTATTAG42800.0265.706766
GGGTTAT43200.0263.92943
GGTTATT43500.0262.448274
TTAGTAG47250.0241.931239
GGGGAGC24900.0239.317263
GGGGGAG25550.0233.806262
CAGAAGC27850.0213.438069
GCAGAAG28850.0205.52868
GGGAGCA29000.0205.482764
AGCAGAA29550.0200.65997
GGAGCAG31200.0190.520845
GAGCAGA31900.0186.340126
GCTCGAT501.6189006E-10177.0295
CCCGGTT401.951541E-6147.49998295
Produced by FastQC (version 0.11.2)