FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b12a_xt_half.3520000003f3eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b12a_xt_half.3520000003f3eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473119
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG13160.27815412190167804No Hit
TAATTATACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT12980.2743495822404089No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA10220.21601330743428188No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTATCACATGTA9020.1906497096924875No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC7960.1682451983539025No Hit
CCATAGAGGCTCTTCATTTGGGTTTTGTTATCCGAATGGAACCCCCAAAC7820.16528611195069318No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG7160.15133613319270628No Hit
TTTTATTACTTTGCTCCTGCCACTCGCGCACCACACCTTTTGAGGCAACA6730.1422475106685633No Hit
GTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTCTTCATGAAGGCAAT6470.13675206449117452No Hit
GTCTCAACTGTACAGATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTG6370.134638431346025No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA6140.12977707511218106No Hit
ATGCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA6020.12724071533800166No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAGTAAGGACAATT5960.12597253545091192No Hit
GCCTCAAGCATGCTATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTT5740.12132254253158298No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACATAC5700.12047708927352314No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC5500.1162498229832241No Hit
ATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTT5490.11603845966870914No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCTTCA5460.1154043697251643No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT5460.1154043697251643No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC5380.11371346320904466No Hit
GGCTTGGGCTGTCCCAAAGGACAACTACAAAAATGCAACGAACCCACAAA5370.1135020998945297No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT5340.11286800995098485No Hit
GATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTGGGGACCACACCTTC5330.11265664663646989No Hit
TTCCTATAATGCACGACAGAACAAAAATCAGGCAACTACCCAATCTTCTC5290.11181119337841008No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC5280.11159983006389512No Hit
GTCTATATTTGGTTCCATTGGCAAGCTTCAAAGGTGTTTTTACCCATATT5120.10821801703165589No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT5090.10758392708811101No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT5070.10716120045908112No Hit
GGGATAACATCTTCAAACTCACCTCATGTGGTCAAAACAGCTACTCAAGG5040.10652711051553625No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG5040.10652711051553625No Hit
GCCCAAGCCATTGTTGCAAAAAATCCGATTTTACTAGTAGCGTTAGGGCA5000.10568165725747644No Hit
ACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACAT4980.10525893062844655No Hit
ATTTATAGCTTCTTGTGTACTCTTAAGGTCTGCCGCCACTGCCACTCCAT4980.10525893062844655No Hit
GTCCCAAAGGACAACTACAAAAATGCAACGAACCCACAAACAGTGGAAGT4960.10483620399941664No Hit
GTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGTAACTGTGT4900.1035680241123269No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA4860.1027225708542671No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTGC1700.0130.147061
GCGGAGC2250.0124.555561
CGGTGCA1250.0118.000012
AGCGGTG703.156117E-584.285711
CGGTGCG703.156117E-584.285712
GGTGCAT2050.071.9512253
AGCACGC2050.071.9512251
GCACGCA2250.065.555562
GTAACAA10650.062.323944
AGCGGAG1206.1445535E-661.4583363
ACGAGCC1554.1872408E-757.0967759
CGCACTT2601.8189894E-1256.7307665
TAGTAAC13500.055.722222
CACGAGC2405.0931703E-1155.3125048
GGTGCGT1102.956862E-453.6363683
TAATTAT8850.046.6666641
GTGCGTT1306.7404576E-445.3846134
ATTATAC9350.044.1711243
CAGGCAG5350.044.112159
TAGTATG12100.043.8842964
Produced by FastQC (version 0.11.2)