FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b12a_xt_full.3520000003f3de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b12a_xt_full.3520000003f3de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548639
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG16340.2978278977615518No Hit
TAATTATACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT12870.23458048006065918No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTATCACATGTA11030.20104294444981125No Hit
GTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGTAACTGTGT9160.1669586011931343No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA8670.15802740964459328No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG8420.15347067926268457No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC8350.15219479475575015No Hit
CCATAGAGGCTCTTCATTTGGGTTTTGTTATCCGAATGGAACCCCCAAAC8170.1489139488807759No Hit
GCCTCAAGCATGCTATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTT7120.1297756812767594No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA7070.12886433520037766No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT7020.1279529891239959No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA6950.1266771046170615No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACAAAAAATCTCAATT6910.1259480277559561No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACATAC6890.12558348932540342No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC6880.12540122011012705No Hit
GTCTCAACTGTACAGATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTG6870.12521895089485072No Hit
ATGCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA6460.11774591306852047No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT6310.11501187483937525No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAGTAAGGACAATT6280.1144650671935462No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCTTCA6250.11391825954771717No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC6230.11355372111716447No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT6230.11355372111716447No Hit
TTTTATTACTTTGCTCCTGCCACTCGCGCACCACACCTTTTGAGGCAACA6140.11191329817967735No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT6130.111731028964401No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC6120.11154875974912465No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC6050.11027287524219022No Hit
GCCCAAGCCATTGTTGCAAAAAATCCGATTTTACTAGTAGCGTTAGGGCA6050.11027287524219022No Hit
ATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTT5880.1071742985824923No Hit
GGCTTGGGCTGTCCCAAAGGACAACTACAAAAATGCAACGAACCCACAAA5770.10516933721445249No Hit
GTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTCTTCATGAAGGCAAT5730.10444026035334711No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG5650.10298210663113633No Hit
GTGTTATTATCACTATATGTGTCAGCTTCATTGTCATACTTACTATATTC5620.10243529898530729No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC5550.10115941447837284No Hit
ACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACAT5540.10097714526309651No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT5500.1002480684019911No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAGC2200.0147.500021
GCGGTGC2000.0110.6251
CGGTGCA1800.0106.527782
AGCGGTG1400.094.821431
AGCACGC2750.085.8181841
GTAACAA12500.083.784
GGTGCAT2450.078.2653053
GCACGCA3100.076.1290362
CGAGCCT2000.073.755
ACGAGCC2300.064.130434
TAGTAAC16900.063.713022
CGCACTT4000.062.68755
CGGTAAG150.00438115159.000004275-279
TAATTAT8200.057.5609741
TAGAAAC5650.057.4336282
ACGCACT4500.055.7222184
CACGCAC4550.055.109893
CACGAGC3850.053.636363
AAACACG5800.053.4051745
ACACGTG2251.7553248E-952.4444437
Produced by FastQC (version 0.11.2)