FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b12a_xt_fourth.3520000003f3f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b12a_xt_fourth.3520000003f3f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282649
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATTATACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT7510.26570056854968527No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG6990.24730319229857525No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA6490.22961340744173867No Hit
CCATAGAGGCTCTTCATTTGGGTTTTGTTATCCGAATGGAACCCCCAAAC5510.19494142912233903No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC5330.18857310657387785No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAGTAAGGACAATT4360.1542549239516149No Hit
GTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTCTTCATGAAGGCAAT4220.14930178419170065No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG4210.14894798849456392No Hit
GTCTCAACTGTACAGATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTG4160.14717901000888026No Hit
GGCTTGGGCTGTCCCAAAGGACAACTACAAAAATGCAACGAACCCACAAA4150.14682521431174353No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTATCACATGTA4130.14611762291747007No Hit
TTTTATTACTTTGCTCCTGCCACTCGCGCACCACACCTTTTGAGGCAACA4030.14257966594610277No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC4000.14151827885469256No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCTTCA3970.14045689176328238No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA3810.13479616060909466No Hit
GCCCAAGCCATTGTTGCAAAAAATCCGATTTTACTAGTAGCGTTAGGGCA3740.13231959072913757No Hit
ATGCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA3680.13019681654631718No Hit
ACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACAT3550.12559747248353964No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC3430.12135192411789889No Hit
TTCCTATAATGCACGACAGAACAAAAATCAGGCAACTACCCAATCTTCTC3430.12135192411789889No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG3370.1192291499350785No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACATAC3280.11604498866084792No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT3260.11533739726657445No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA3230.11427601017516426No Hit
ATTTATAGCTTCTTGTGTACTCTTAAGGTCTGCCGCCACTGCCACTCCAT3230.11427601017516426No Hit
GTCCCAAAGGACAACTACAAAAATGCAACGAACCCACAAACAGTGGAAGT3200.11321462308375406No Hit
GTACAAATGTATGGTACTTCCACTGTTTGTGGGTTCGTTGCATTTTTGTA3070.10861527902097654No Hit
GGGATAACATCTTCAAACTCACCTCATGTGGTCAAAACAGCTACTCAAGG3060.10826148332383982No Hit
GATCTGGATGTGGCCTTGGGCAGGCCAATGTGTGTGGGGACCACACCTTC3060.10826148332383982No Hit
ATCCAATGCAGATCGAATCTGCACTGGGATAACATCTTCAAACTCACCTC3040.10755389192956635No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTCTTCATGAAGGCA2930.1036621392610623No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT2920.10330834356392557No Hit
TTATACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGG2910.10295454786678886No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC2890.10224695647251539No Hit
GCCTCAAGCATGCTATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTT2880.10189316077537866No Hit
ATTAAAGGCAATGACCCTTTTATTACTTTGCTCCTGCCACTCGCGCACCA2830.100124182289695No Hit
ATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTT2830.100124182289695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTGC750.0177.01
GGTGCAT750.0157.333343
CGGTGCA854.7293724E-11121.470592
GCACGCA1301.3606041E-979.423082
CGCACTT1301.3606041E-979.423085
AGCACGC1402.4483597E-973.751
ACGAGCC1052.778841E-670.23819
GTTAAGG901.0956586E-465.555561
GCGGAGC901.0956586E-465.555561
TAGAAAC2850.062.1052672
AGCGGAG951.4317161E-462.105261
GTAACAA7050.060.673764
CAGGCAG3100.057.0967759
CGCTATT850.007182355552.058823295
CCGAAAA850.007182355552.058823295
TAGTAAC9000.050.8055532
CACGAGC1759.662836E-750.571438
TAACAAG9100.048.6263735
TCGCCCC1255.5519753E-447.2295
CACGTGC1603.3698E-546.093758
Produced by FastQC (version 0.11.2)