FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_b12a.3520000003f52f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_b12a.3520000003f52f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206740
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA31321.514946309374093No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATCAAGTTCAG29961.4491632001547838No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA25551.2358517945245235No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC24771.1981232465899196No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC19350.9359582083776725No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT17160.8300280545612847No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTCATT15270.7386088807197446No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT14360.6945922414627068No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAACAAGA14280.6907226468027474No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTATAT12410.6002708716261972No Hit
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT12360.5978523749637226No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTT10590.5122375931121215No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCGTATTTTCT9440.4566121698752056No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA9060.43823159524039856No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATCAAGTTCAG8780.4246880139305408No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTATAT7990.38647576666344197No Hit
GGGGGGAGCAGAAGCAGAGGATTATGATGACCTCGATTATTAAAGCAACA7870.3806713746735029No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC7150.34584502273386863No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCC6680.32311115410660735No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTT6480.3134371674567089No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTATATTCATTG5890.2848989068395086No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCGTATTTTCT5320.25732804488729805No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCTAAAATTGAA5060.2447518622424301No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTT4830.2336267775950469No Hit
GGGGGGAGCAGAAGCGGTGCATTTTCTAATATCCACAAAATGAAGGCAAT4440.21476250362774502No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC4370.21137660830028054No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCC3800.18380574634807004No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCTAAAATTGAA3590.1736480603656767No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTATATTCATTG2930.1417239044210119No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTTATATTTATTTTAACAAGA2510.1214085324562252No Hit
GGGGGAGCAGAAGCGGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCA2500.12092483312373029No Hit
GGGGGGAGCAGAAGCGGAGCGTTTGATTTGTCATAATGGATACTTTCATT2200.10641385314888266No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCACTCAATTATATTCATTG2110.10206055915642837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTT22350.0291.700231
CGGGTTA22750.0287.21982
ATTAGTA23950.0272.82888
TATTAGT23950.0272.212927
TTATTAG25050.0259.670656
GGGGGGA18700.0257.92781
GGGTTAT26000.0250.18273
GGAGCAG19600.0246.084185
GTTATTA26600.0244.539475
TTAGTAG26800.0243.81539
GGGGAGC19900.0243.11563
GAGCAGA19900.0243.11566
CAGAAGC20200.0240.965339
GGTTATT28600.0227.438834
GGGAGCA22100.0217.57924
GCAGAAG24500.0198.071438
GGGGGAG24900.0193.704822
AGCAGAA27300.0178.29677
GGGTTAC508.760537E-488.53
CAAGGTT703.1519023E-584.28571295
Produced by FastQC (version 0.11.2)