FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a97.3520000003f661.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a97.3520000003f661.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257273
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA131965.129181841856704No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC110554.296991911315995No Hit
CGGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTC87083.3847313942776744No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG81423.1647316274929747No Hit
GGGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT71602.7830359190431952No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA68942.679643802497736No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA67562.6260042833876853No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG65372.54088069871304No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT45091.7526129830957777Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT41471.6119064184737613No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA40671.5808110450766306No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG30301.1777372674163242No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG29341.1404228193397676No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT24550.9542392711244475No Hit
GGGGGGAGCAAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT24240.9421898139330593No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG22430.8718365316220512No Hit
CGGGTTATTAGTAGAAACAAGGTCTGTATTCGTACTGAAAATCAAGATCA17660.6864303677416597No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT16640.646783766660318No Hit
GGGGGGAGCGAAAGCAGGCGAGCTACCTAAGAACAGCTAAAAGAGCACAC16080.6250170052823266No Hit
GGGGGGAGCGAAAGCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC15880.617243161933044No Hit
GGGGGGAGCGAAAGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT15230.5919781710478752No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA14840.576819176516774No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAAAGAGGAACAGCTCTTTGGACAC14490.5632149506555294No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG14410.5601054133158163No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT13090.5087980472105507No Hit
CGGGTTATTAGTAGAAACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTC12410.48236697982298965No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC11820.45943414194260573No Hit
GGGGGGAGCGAAAGCAGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAG11330.4403882257368632No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG9740.3785861711100659No Hit
CGGGTTATTAGTAGAAACAAGGCGCCCGTCACGCACCGCACGTTCGTGGG8470.329222265842121No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA8330.3237805754976232No Hit
CGGGTTATTAGTAGAAACAAGGCTGTTAATTGTCAGTTCAGTGTTTTAAT8170.317561500818197No Hit
GGGGGGAGCGAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACC6990.2716958250574293No Hit
GGGGGGAGCGAAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC6410.24915167934450952No Hit
GGGGGGAGCAAAAGCAGGCGAGCTACCTAAGAACAGCTAAAAGAGCACAC5130.19939908190910044No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT4860.18890439338756887No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT4730.18385139521053512No Hit
GGGGGGAGCAAAAGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT4550.1768549361961807No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4530.17607755186125246No Hit
GGGGGGAGCAACAGCTATACTAAGAAAAGCAACCAGAAGATTGGTTCAAC4160.1616959416650795No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT3980.15469948265072508No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA3960.15392209831579684No Hit
GGGGGGAGCAAAACCAGAAGAAGTGTCATTCCGGGGGAGGGGAGTTTTCG3680.1430387176268011No Hit
GGGGGGAGCAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACC3650.1418726411244087No Hit
GGGGGGAGCAAAAGCAGGCCGGGCGCGACCCGCTCCGGGGACAGTGCCAG3510.13643095077991083No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT3400.13215533693780535No Hit
GGGGGGAGCGAAAGCAGGGCGACAAACCTACCGAGCCTGGTGATAGCTGG3350.13021187610048468No Hit
GGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAA3230.1255475700909151No Hit
CGGGTTATTAGTAGAAACAAGGCAGCCACCCGAGATTGAGCAATAACAGG3150.12243803275120203No Hit
GGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGC3090.12010587974641722No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATAT2970.11544157373684764Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT2850.11077726772727803No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACA2610.10144865570813882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT69300.0285.209232
GTTATTA69100.0283.900154
TTATTAG69200.0283.703035
GGTTATT69450.0283.318943
GGGGGGA65850.0282.904331
TTAGTAG69550.0281.85128
TATTAGT70300.0279.473666
ATTAGTA70700.0277.683877
TAGTAGA71700.0272.988169
GGGGAGC68900.0267.597963
GGGGGAG70100.0265.331672
GGAGCGA51600.0264.413765
CGAAAGC51900.0262.316969
GGGAGCG52400.0260.37694
GAGCGAA52950.0257.95096
GCGAAAG53150.0256.980228
AGCGAAA53150.0256.980227
CGGGTTA79150.0249.343021
GGGAGCA20300.0233.238924
GCAAAAG18500.0219.256768
Produced by FastQC (version 0.11.2)