FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a90.3520000003f655.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a90.3520000003f655.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242847
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC156666.450975305439227No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG76263.1402487986262955No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA73973.0459507426486634No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT57062.349627543267984No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA44991.8526067853422115No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG41661.715483411366004No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT34121.4049998558763337Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT33241.3687630483390776No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT33101.3629981016854233No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT23600.9718052930445917No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTTGGACAGTACGGATGACAA22120.9108615712773888No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA17860.7354424802447632No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG17410.7169122945723028No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT16960.6983821088998423No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG15740.6481447166322829No Hit
GGGGGGAGCGAAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATC12040.49578541221427486No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG10810.44513623804288294No Hit
GGGGGGAGCGAAAGCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC10000.41178190383245417No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA9980.4109583400247893No Hit
CGGGTTATTAGTAGAAACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT9810.40395804765963755No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7530.310071773585838No Hit
GGGGGGAGCAAAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATC7010.28865911458655036No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6230.25654012608761895No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5120.21083233476221655No Hit
GGGGGGAGCAAAACCAGAAGAAGTGTCATTCCGGGGGAGGGGAGTTTTCG4950.20383204239706482No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC4870.20053778716640516No Hit
GGGGGGAGCGAAAGCAGGAGTGGATAGATTCTATAGGACCTGCAAGTTAG4530.18653720243610175No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCT4380.18036047387861492No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA4280.1762426548402904No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA4090.16841879866747375No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3950.1626538520138194No Hit
GGGGGGAGCGAAAGCAGGCAGCAGAGGCCATGGAGATTGCTAGTCAGGCC3680.15153574061034314No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACA3640.14988861299501333No Hit
GGGGGGAGCAACAGCTATACTAAGAAAAGCAACCAGAAGATTGGTTCAAC2500.10294547595811354No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA2430.10006300263128637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA47900.0291.304781
GGGGGGA38400.0285.397161
GGGTTAT49200.0283.607732
GGTTATT49750.0279.582923
GTTATTA49550.0279.52074
TTATTAG49900.0276.673345
GGGGAGC39700.0271.59323
TATTAGT51000.0270.705876
TTAGTAG51000.0270.416668
CGAAAGC29900.0267.86799
ATTAGTA51500.0267.791267
GGAGCGA30150.0265.157535
GGGAGCG30300.0264.331674
TAGTAGA53000.0260.76899
GCGAAAG31350.0255.007988
GAGCGAA31700.0251.727136
GGGGGAG43400.0250.817982
AGCGAAA31900.0250.148917
GGGAGCA11850.0232.76374
GCAAAAG13700.0174.416068
Produced by FastQC (version 0.11.2)