FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a89.3520000003f505.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a89.3520000003f505.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285153
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT111623.9143898187990307No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA97663.4248280747528517No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC79792.7981469597023354No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA69602.440794941662897No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT51331.8000862694763864No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG41951.471140054637335No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT38721.357867530764186No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG37901.3291110386353993No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT36871.2929900790102156Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG27140.9517697516771698No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG24550.8609413192216109No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA20140.7062875017972808No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT19930.6989230343008841No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT16580.5814422432869372No Hit
CGGGTTATTAGTAGAAACAAGGGGATTTATAGGTAGAGGCGACAAACCTA13330.46746834155698874No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA12820.4495832062085968No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA10940.3836536876694266No Hit
GGGGGGAGCGAAAGCAGGCTTATGCGGAGGAGAATGTTTTCATGTTACTT10070.3531437508986404No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT8980.31491865770305766No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8750.3068528123498613No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG6700.2349615820278938No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG5000.17534446419992075No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT3940.13817143778953753Illumina Single End PCR Primer 1 (96% over 32bp)
GGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTTC3870.13571661529073867No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT3590.1258973252955431No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3420.1199356135127458No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGGAAGCTTAT3260.11432459065834832No Hit
GGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAAT2950.10345323387795323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA50150.0291.76471
GGGTTAT51300.0284.074072
GGTTATT51950.0279.384033
GTTATTA51750.0279.323674
GGGGGGA34050.0278.53891
TTAGTAG52350.0275.276988
GGGGAGC34150.0274.267943
TTATTAG53000.0272.735845
ATTAGTA53000.0272.457557
GGAGCGA27900.0272.267035
TATTAGT53250.0270.90146
GGGAGCG28150.0270.373024
CGAAAGC27350.0270.191969
TAGTAGA54000.0266.59269
GAGCGAA28550.0262.451846
GCGAAAG28200.0262.047858
AGCGAAA29450.0253.930397
GGGGGAG42100.0225.279112
AAACGTT1400.0168.57143295
CGGGCTA301.1481787E-4147.499981
Produced by FastQC (version 0.11.2)