FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a81.3520000003f4f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a81.3520000003f4f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249304
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA165156.624442447774605No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA116524.673811892308186No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA58642.3521483810929626No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC58162.3328947790649166No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG41071.6473863235246928No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA33551.3457465584186374No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT31351.2575008824567597No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT26011.043304559894747No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT23220.9313929981067292No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT22470.9013092449379071No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT20030.8034367679620062Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG19330.7753585983377723No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT18670.748884895549209No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT17580.7051631742771877No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG14940.5992683631229343No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA14380.5768058274235471No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG13980.5607611590668421No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA11260.45165741424124767No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA9690.3886820909411802No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9130.3662195552417931No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7970.31969001700734845No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6720.26955042839264515No Hit
GGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAA4970.19935500433206046No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG4850.19454160382504893No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4750.19053043673587267No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA4440.17809581875942623No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA4350.1744857683791676No Hit
GGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAAT4000.16044668356705066No Hit
GGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAAT3540.14199531495683984No Hit
GGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATG3390.13597856432307542No Hit
AAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAAC3160.12675288001797003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA41700.0289.34051
GGGTTAT42450.0284.923432
GTTATTA43450.0277.008034
GGGGGGA40900.0275.88631
GGTTATT44600.0271.188323
GGGGAGC41450.0270.44633
GGGAGCG32200.0270.263984
TTATTAG44600.0270.19625
TTAGTAG45100.0267.52778
GGAGCGA32650.0266.99085
TATTAGT45650.0263.981356
ATTAGTA45950.0261.93697
TAGTAGA46100.0261.08469
GAGCGAA34600.0251.517336
GGGGGAG45050.0249.489462
AGCGAAA35550.0244.381157
CGAAAGC35700.0243.767529
GCGAAAG37000.0235.601358
GGGAGCA11100.0227.229724
TTAAGCC750.0196.66667295
Produced by FastQC (version 0.11.2)