FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a8.3520000003f420.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a8.3520000003f420.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200862
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA98074.88245661200227No Hit
CGGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTC83824.173014308331093No Hit
GGGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT78743.920104350250421No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG66133.292310143282453No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC59062.9403271898119105No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG41682.0750565064571695No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT37331.858489908494389No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT28011.3944897491810297No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT21401.0654080911272417No Hit
GGGGGGAGCAAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT18100.9011161892244426No Hit
CGGGTTATTAGTAGAAACAAGGGGCAGGGACTTAATCAACGCAAGCTTAT17130.8528243271499836No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT16180.8055281735719051No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA15970.7950732343599088No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG15820.7876054206370543No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT15580.775656918680487Illumina Single End PCR Primer 1 (97% over 34bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG14810.737322141569834No Hit
GGGGGGAGCGAAAGCAGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCC14380.7159144088976511No Hit
GGGGGGAGCGAAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC12940.644223397158248No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA10210.5083091874022961No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA9960.4958628311975386No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT9620.47893578675906845No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTT8490.4226782567135645No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA8300.41321902599794885No Hit
CGGGTTATTAGTAGAAACAAGGGACCGGGGTCCGGTGCGGAGTGCCCTTC7920.39430056456671747No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA7920.39430056456671747No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7670.38185420836195993No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG6920.3445151397476875No Hit
GGGGGGAGCGAAAGCAGGTTTCAGGTTACAAAGTAAAATCACCATCCGAT6510.32410311557188515No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT5570.277304816241997No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5200.25888420905895587No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT4630.2305065169121088No Hit
GGGGGGAGCAAAAGCAGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCC4440.2210472861964931No Hit
GGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTT3790.18868676006412363No Hit
CGGGTTATTAGTAGAAACAAGGGGGACCGGGGTCCGGTGCGGAGTGCCCT3490.17375113261841463No Hit
CGGGTTATTAGTAGAAACAAGGGCTAATTCTATGGTTTCTGAAAATGTGA3200.15931335942089594No Hit
CGGGTTATTAGTAGAAACAAGGGCTAATTCAGGCAGAAATTCAAAATGGA3090.15383696269080266No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT2650.13193137577042943No Hit
GGGGGGAGCAAAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCC2600.12944210452947794No Hit
GGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTCA2590.12894425028128764No Hit
CGGGTTATTAGTAGAAACAAGGCGGGACCGGGGTCCGGTGCGGAGTGCCC2570.12794854178490705No Hit
GGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGC2470.12296999930300405No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTT2430.12097858231024286No Hit
GGGGGGAGCAACAGCTATACTAAGAAAAGCAACCAGAAGATTGGTTCAAC2410.11998287381386225No Hit
GGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAA2390.11898716531748166No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT2200.10952793460186595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT45550.0280.42812
GGGGGGA43100.0279.942021
TTATTAG46150.0274.225345
GTTATTA46450.0274.0424
TTAGTAG46600.0273.79298
GGTTATT47050.0271.17433
TAGTAGA47250.0269.40219
GGGGAGC44400.0269.087833
GGGAGCG35750.0268.594424
ATTAGTA47800.0266.61097
GGAGCGA36150.0266.438455
TATTAGT48150.0264.979256
GAGCGAA37350.0256.693456
CGAAAGC38450.0251.267879
AGCGAAA38300.0250.711477
GGGGGAG48500.0248.164952
GCGAAAG40250.0239.298138
GGGAGCA9800.0236.301034
CGGGTTA58000.0220.487061
CTAGCGC255.549714E-5177.0295
Produced by FastQC (version 0.11.2)