FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a78.3520000003f4ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a78.3520000003f4ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285209
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC195426.851817439141121No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA138394.852231170825605No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG84952.978517508213275No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG73322.5707463649464075No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT57612.0199222324681196No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT53301.8688049816099772No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG37721.3225389100624454No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT37601.318331469203286No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA34651.2148985480822836No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT33671.1805377810658149Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA18540.6500496127401308No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA18260.6402322507354256No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT18120.6353235697330729No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT17060.5981578421438314No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG15480.5427598708315656No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA15330.5375005697576163No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG14740.5168139855334158No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7630.26752311462822No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5850.20511274188402187No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACA4900.17180383508234312No Hit
CGGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTC4680.1640901935072175No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT4240.1486629103569663No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT4170.14620856985578995Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT3910.1370924479942779No Hit
GGGGGGAGCGAAAGCAGGCAGCGGAGGCCATGGAGATTGCTAGTCAGGCC3790.13288500713511847No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA3740.13113190677713538No Hit
GGGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT3600.1262232257747827No Hit
GGGGGGAGCGAAAGCAGGAGTGGATAGATTCTATAGGACCTGCAAGTTAG3480.12201578491562329No Hit
GGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAA3410.11956144441444695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA56150.0290.796971
GGGTTAT57500.0283.713042
GGGGGGA38950.0283.26061
GTTATTA57400.0282.151554
GGTTATT57750.0281.718633
TTATTAG58350.0277.557835
TTAGTAG58000.0277.452588
ATTAGTA58700.0275.149087
TATTAGT58700.0274.89786
GGGGAGC40450.0270.933233
GGGAGCG33850.0270.598244
GGAGCGA33850.0269.726755
TAGTAGA59700.0268.810739
CGAAAGC33800.0267.0719
GAGCGAA34550.0261.273536
GCGAAAG35200.0256.448888
AGCGAAA35200.0256.448887
GGGGGAG45000.0246.16112
GGGAGCA8400.0215.982154
GCAAAAG11950.0141.945628
Produced by FastQC (version 0.11.2)