FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a57.3520000003f4dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a57.3520000003f4dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282222
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG189736.722721828914826No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC120004.25197185194634No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA117484.162680443055467No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT92243.2683490301960867No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG70052.482088568573676No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA67322.3853562089418965No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT57142.0246472635017825No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA45631.616812296702596No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA39741.4081113449695628No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT37271.3205915910170007No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT28791.0201189134794595Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT28301.0027566950840119No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA25250.8946857438470424No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT22110.7834258137211132No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA22020.7802368348321534No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT19630.6955517287808888No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT15880.5626776084075658No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG14280.5059846503816144No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA9790.3468900369212889No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA7510.26610257173430846No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6850.2427167265486036No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT6800.2409450716102926No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6470.22925214901744015No Hit
CGGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTC6330.22429151519016946No Hit
GGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGA4940.17503950790512432No Hit
GGGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT4290.15200799370708168No Hit
GGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAA4050.143504050003189No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT3230.114448909014889Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC3120.11055126815060483No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACA2890.10240165543437436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA68050.0290.44821
GGGTTAT68900.0286.436862
GGGGGGA54400.0285.51011
TTATTAG69250.0282.43325
GTTATTA69450.0281.19514
TTAGTAG69700.0279.551648
GGTTATT71150.0275.92763
GGGGAGC56500.0274.115053
TATTAGT71300.0273.899026
GGAGCGA43600.0272.33375
ATTAGTA71750.0271.770057
GGGAGCG43950.0270.164954
TAGTAGA73450.0265.479929
GGGGGAG58700.0264.846682
AGCGAAA45550.0260.99897
GAGCGAA45500.0260.637366
CGAAAGC45900.0259.972759
GCGAAAG46650.0255.160778
GGGAGCA14450.0247.024234
TTAGTCG202.280301E-5221.258
Produced by FastQC (version 0.11.2)