FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a39_xt_half.3520000003f3b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a39_xt_half.3520000003f3b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514544
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA11770.2287462296713206No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGGAAAAAAGACG9260.17996517304642556No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA8060.15664355234926458No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT7850.15256226872726142No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT7850.15256226872726142No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG7770.15100749401411737No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA7260.14109580521782392No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC7210.1401240710221089No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG7040.13682017475667776No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA6680.12982368854752946No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA6550.12729717963867038No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT6500.12632544544295532No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG6470.1257424049255263No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT6380.12399328337323921No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT6340.12321589601666717No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA6180.12010634659037907No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT6140.11932895923380703No Hit
GTCCCAGATATGTTAAGCATAGCACTCTGAAATTGGCAACAGGAATGCGA5850.11369290089865977No Hit
TCCCTGTACACACTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA5580.10844553624179856No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA5540.10766814888522652No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTTCAAACAG5510.1070851083677975No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG5440.10572468049379645No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA5430.10553033365465345No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT5410.10514163997636743No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA5380.1045585994589384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG3050.096.721319
GCAGGGT2650.083.490569
TACGGAT2350.075.319152
AAGCAGG19200.074.5182347
AGCAGGT10550.072.701428
CAAAAGC20000.069.3254
GCAAAAG21050.063.0641333
AGGGTTA2850.062.105269
GCTATAA5000.059.01
CGGATAA3200.055.31254
GCTAAAT3100.052.338717
AGCAAAA26050.051.5259132
CGAAAGC2905.456968E-1250.862073
AAGCTAA3200.050.7031255
ACGGATA3500.050.571433
TAACTAT6250.049.5599985
GAGCAAA27750.047.306311
GGTCCGC1952.0400603E-645.3846178
TATAACT6700.044.029853
CTAAATT3701.8189894E-1243.8513538
Produced by FastQC (version 0.11.2)