FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a39_xt_full.3520000003f3a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a39_xt_full.3520000003f3a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613766
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA16980.27665266567388874No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGGAAAAAAGACG12100.19714353678763566No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT11450.18655318150565525No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG10770.17547404059527572No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA10020.16325439988529833No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG9170.1494054737473239No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT9010.14679861706252872No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC8520.13881511846534347No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA8490.13832633283694437No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT8320.1355565476093495No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA8150.13278676238175463No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA8090.13180919112495643No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG7500.12219640709977418No Hit
GTCCCAGATATGTTAAGCATAGCACTCTGAAATTGGCAACAGGAATGCGA7410.12073005021457689No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA7020.11437583704538862No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA7010.11421290850258893No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT6910.11258362307459194No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT6830.11128019473219436No Hit
CCCTTATTCTGGGTCTAGATCCGATATTTGGGATTACAGCTTGTTGGCTC6760.11013969493259645No Hit
TCCCTGTACACACTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA6690.10899919513299858No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT6440.1049259815630061No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG6380.10394841030620791No Hit
GAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCT6310.10280791050661No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGT3400.0121.47069
GCAGGGG3900.0121.025649
AAGCAGG26750.080.504687
CAAAAGC26900.075.669144
GCAAAAG26850.075.260713
AGCAGGT14500.069.172428
CGAAAGC3650.064.657533
GCTAAAT3250.063.5384647
AGCAAAA32150.063.3125952
AAGCTAA3350.061.6417925
GAGCGAA3500.058.9999961
AGCAGGG15200.058.2236828
GAGCAAA36600.056.017761
CTAAATT3700.055.810818
AAAGCAG44200.050.7239846
CAAGCTA4100.050.3658564
CTAGAGT4700.047.0744676
AGCGAAA5350.046.8691561
GCTATAA4450.046.404491
AGGGTTA3250.045.3846179
Produced by FastQC (version 0.11.2)