FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a39_xt_fourth.3520000003f3c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a39_xt_fourth.3520000003f3c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239367
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5380.22475946976818023No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA3900.1629297271553723No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGGAAAAAAGACG3880.16209419009303705No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT3870.16167642156186943No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG3780.15791650478136082No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG3660.15290328240734938No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT3410.14245906912815884No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTTCAAAATAA3150.1315970873178007No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC3140.13117931878663308No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA3100.1295082446619626No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT3050.1274194020061245No Hit
ACCATTTCCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTT3010.12574832788145401No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT2910.12157064256977779No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA2820.11781072578926921No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2810.11739295725810157No Hit
TCCCTGTACACACTGTGGTCATAAGTTCCATTTCTTATTGATCCTATGCA2780.11613965166459872No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA2780.11613965166459872No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG2770.11572188313343108No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTTTTTCAAACAG2690.11237973488409012No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT2610.10903758663474916No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2570.10736651251007866No Hit
GTCCCAGATATGTTAAGCATAGCACTCTGAAATTGGCAACAGGAATGCGA2550.10653097544774343No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG2530.1056954383854082No Hit
GCTATAACTATTTTAATGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA2440.1019355216048996No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGG900.0147.59
GAGCGAA1601.8189894E-1282.968741
AAGCAGG8200.082.743917
GCAGGGT901.1111078E-681.944457
AGGGTGT806.112587E-573.749996
AGGGTTA901.09531684E-465.555569
AGCAGGT5000.064.98
CGAAAGC2051.0913936E-1164.75614
GCAAAAG8800.063.693183
CAAAAGC9200.059.3206524
AGCGAAA2252.7284841E-1158.9999962
GGGTTAA751.5919795E-758.9999961
CGTGATA1801.7831553E-857.3611154
TAGAAAC3150.056.1904752
GTAGAAA3250.054.4615361
ACGTGAT1953.3525794E-852.948723
AGCAAAA11650.051.909872
TACGTGA2004.09309E-851.6252
GATAGGA2054.9720256E-850.3658567
CCTATAG2352.568413E-950.2127653
Produced by FastQC (version 0.11.2)