FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a39.3520000003f4c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a39.3520000003f4c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199626
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC51542.5818280183943974No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG33961.7011812088605693No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA32791.6425716089086593No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT32141.6100107200464868No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT22731.1386292366725776No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA21861.0950477392724394No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT21371.0705018384378788Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT20981.0509653051205754No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT19380.9708154248444592No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG19080.9557873222926873No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT15890.7959884984921805No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT14980.7504032540851392No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG11840.5931091140432608No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA11300.5660585294500716No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA11240.5630529089397173No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT7850.3932353501046958No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA7650.38321661507018123No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT6270.3140873433320309No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA6070.30406860829751636No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG4450.22291685451794854No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4400.2204121707593199No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA4280.2144009297386112No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA4100.20538406820754812No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA3240.16230350755913558No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCCCTATGTGTAGATCT2040.10219109735204832Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA22300.0291.031371
GGGTTAT23350.0277.944342
GGGAGCG15250.0270.819674
GGAGCGA15300.0269.934665
GGGGAGC20000.0269.925023
GGGGGGA20400.0266.078431
GTTATTA24300.0265.86424
GGTTATT24750.0261.03033
TTAGTAG25600.0253.515628
TATTAGT25550.0253.434456
TTATTAG25500.0253.352925
ATTAGTA26300.0246.768077
TAGTAGA26750.0243.168239
CGAAAGC17150.0242.536449
GAGCGAA17700.0234.999986
AGCGAAA17750.0234.338047
GCGAAAG18200.0228.543968
GGGGGAG23850.0226.970662
GGGAGCA6050.0212.107424
CGGGCTG200.006733555147.5295
Produced by FastQC (version 0.11.2)