FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a3.3520000003f413.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a3.3520000003f413.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209969
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATCAATGCACTCAAATGCA90294.300158594840191No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC62162.96043701689297No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG47242.2498559311136406No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG42972.0464925774757226No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT36551.7407331558468155No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT25321.2058922983869047No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA22111.0530125875724512No Hit
CGGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTC21681.0325333739742533No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT21621.0296758092861327No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT21081.003957727093047Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT20380.9706194723983064No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA20050.954902866613643No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG19160.912515657073187No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT14870.7081997818725622No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA12020.5724654591868323No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA11810.5624639827784101No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG11280.537222161366678No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG11250.5357933790226177No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT10510.5005500812024632No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT9910.4719744343212569No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG8230.39196262305387936No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT5800.276231253184994No Hit
GGGGGGAGCAAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT5510.26241969052574426No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA4880.2324152613004777No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA4430.21098352613957297No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT3830.1824078792583667No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG3220.153355971595807No Hit
CGGGTTATTAGTAGAAACAAGGCCCCAACTTCTTAGAGGGACAAGTGGCG2710.12906667174678166No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT2490.11858893455700603No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA2340.11144502283670446No Hit
GGGGGGAGCAACAGCTATACTAAGAAAAGCAACCAGAAGATTGGTTCAAC2330.11096876205535103No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC2270.1081111973672304No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA2260.10763493658587696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT32650.0287.320042
GTTATTA33650.0277.028234
GGTTATT33850.0276.262943
GGGGGGA32350.0275.850071
TTATTAG33800.0275.798835
CGGGTTA35150.0267.30441
GGGGAGC33500.0265.940283
TTAGTAG35350.0263.705788
TATTAGT35550.0262.22226
GGGAGCG26450.0261.540654
ATTAGTA35700.0261.120457
GGAGCGA26350.0260.85395
CGAAAGC27000.0251.842599
TAGTAGA37400.0249.251339
GAGCGAA27800.0245.656486
GGGGGAG36750.0243.625852
AGCGAAA28600.0238.269237
GCGAAAG29200.0232.868158
GGGAGCA9200.0205.217394
GCAAAAG10600.0166.981128
Produced by FastQC (version 0.11.2)