FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a29.3520000003f648.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a29.3520000003f648.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237547
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA162646.846645084972658No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG141195.943665885066955No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG129455.449447898731619No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA90623.8148240137741167No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT80533.3900659658930654No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA74713.1450618193452238No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC67862.8566978324289507No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG50312.1178966688697396No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT47231.9882381170884078No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA42121.7731227925421076No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG37241.5676897624470105No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT31611.3306840330545113Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA23700.9976972977979094No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA20170.8490951264381364No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT15990.6731299490206148No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT15260.6423991883711434No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT14930.6285072006802864No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG14030.5906199615234038No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG12770.5375778267037681No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA12100.5093728819980888No Hit
GGGGGGAGCGAAAGCAGGTCAATTAAATTCAGTATGGAAAGAATAAAAGA10930.46011947109414136No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT10390.4373871276000118No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA8570.3607707106383158No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT6160.2593171035626634No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG4960.20880078468681987No Hit
GGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGC4910.20669593806699307No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAGCTGAAATGAG4680.1970136436157897No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC3770.1587054351349417No Hit
GGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGA3620.1523908952754613No Hit
GGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAA3380.14228763150029256No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT3000.12629079718960878No Hit
GGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAA2890.1216601346259898No Hit
GGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTGCC2840.11955528800616298No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA2810.11829238003426691No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATAT2460.1035584536954792Illumina Single End PCR Primer 1 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA64800.0291.13041
GGGGGGA55250.0287.791871
GGGTTAT66450.0283.45752
TTATTAG66900.0280.448435
GGTTATT67600.0277.980773
TTAGTAG67300.0277.685738
GTTATTA67700.0277.134434
TATTAGT67600.0277.1086
ATTAGTA68050.0274.842047
TAGTAGA69500.0268.683449
GGGGAGC59500.0267.23533
GGGGGAG60150.0264.347442
GGAGCGA46600.0262.398075
GGGAGCG46500.0262.010744
GAGCGAA47800.0255.193536
AGCGAAA47950.0254.395227
CGAAAGC48850.0249.70839
GCGAAAG49400.0246.928138
GGGAGCA15700.0234.872624
CAAAAGC17000.0209.97069
Produced by FastQC (version 0.11.2)