FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a252.3520000003f67e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a252.3520000003f67e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210317
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC93794.4594588169287315No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG60662.88421763338199No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA49232.340752292967283No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCCTTAATTGTCGTACTCT42422.016955357864557No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT37721.7934831706424113Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT24241.1525459187797467No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG19760.9395341318105526No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT16930.8049753467384948No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT16090.7650356366817709No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA15890.7555261819063605No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG14580.693239253127422No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA13790.6556769067645507No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA10460.49734448475396664No Hit
GGGGGGAGCGAAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATC9750.46358592030125956No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA9130.4341066104974871No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8940.4250726284608472No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT6620.314762953066086No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA6390.30382708007436393No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5790.2752987157481326No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT5120.24344204225050758No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA4160.19779665932853738No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACA3130.14882296723517358No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA2930.13931351245976312No Hit
GGGGGGAGCAAAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATC2800.1331323668557463No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT2720.12932858494558214No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT2430.11553987552123698No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG2270.10793231170090863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA29200.0291.969181
GGGTTAT30300.0278.935642
TTATTAG31050.0271.723055
GTTATTA31050.0271.2484
GGGGGGA22800.0270.41671
GGTTATT31550.0267.41683
TTAGTAG32000.0264.11728
GGGAGCG18500.0259.12164
GGAGCGA18500.0256.729745
ATTAGTA32750.0256.717567
GGGGAGC23750.0256.494753
TATTAGT32800.0256.32626
TAGTAGA33350.0253.868079
CGAAAGC19350.0245.452189
GAGCGAA20100.0234.825876
AGCGAAA20200.0233.663387
GCGAAAG21000.0226.166678
GGGGGAG27300.0225.30222
CTTCCGA150.0028480485196.666661
GGGAGCA6850.0185.182484
Produced by FastQC (version 0.11.2)