FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a24.3520000003f44a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a24.3520000003f44a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188070
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC80714.291487212208221No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG53892.8654224490881055No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT38442.0439198170893813No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA29551.5712234806189183No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA29001.5419790503535917No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG28581.5196469399691603No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT21441.1400010634338278No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT19941.060243526346573No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA16750.890625830807678No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT16160.8592545328866911No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT13580.722071569096613No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA13220.7029297601956719No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT12090.6428457489232733No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT11880.6316796937310576Illumina Single End PCR Primer 1 (97% over 34bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA10470.5567076088690381No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG9110.48439410857659376No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA5550.29510288722284256No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5190.2759610783219014No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT3740.1988621258042218No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT3650.19407667357898656No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA3010.1600467910884245No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACA2390.12708034242569255No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT2310.12282660711437231No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA2130.11325570266390174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA28050.0292.89661
GGGTTAT28550.0286.73382
GTTATTA29150.0279.313874
GGGGGGA20700.0277.898561
TTATTAG29800.0273.221475
GGTTATT30000.0272.8753
TTAGTAG30250.0270.61988
ATTAGTA30400.0268.799327
TATTAGT30500.0267.434426
GGGGAGC21700.0265.092163
GGAGCGA18350.0257.22075
GGGAGCG18300.0257.11754
TAGTAGA31950.0254.835689
AGCGAAA18900.0246.613757
GAGCGAA19250.0243.662346
GGGAGCA4350.0237.356344
CGAAAGC19700.0234.35289
GGGGGAG24850.0231.488942
GCGAAAG21100.0220.201428
CGGGCTA150.00284781196.666661
Produced by FastQC (version 0.11.2)