FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a20.3520000003f5a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a20.3520000003f5a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244780
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC67632.762889124928507No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG50402.058991747691805No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG45761.8694337772693848No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCCT45191.8461475610752514No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA39591.6173707002206064No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA32741.3375275757823353No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT32401.323637552087589No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT31951.3052536971974833No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA29921.2223220851376746No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGAACAAGCTAAATT29111.1892311463354848No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT22120.9036686003758476Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTT21880.8938638777677915No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA18980.7753901462537789No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG17780.7263665332134979No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT11130.4546940109486069No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT10720.4379442764931776No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA10580.4322248549718114No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT9010.368085627910777No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA6820.2786175341122641No Hit
GGGGGGAGCGAAAGCAGGAGTGAAGATGAATCCAAATCAAAAGATAATAA5890.24062423400604624No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTT5110.20875888552986355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA29150.0291.9641
GGGTTAT29500.0287.52
GGTTATT29950.0283.18033
GTTATTA30350.0279.448124
TTATTAG31150.0274.165345
GGGGAGC33250.0264.834563
TTAGTAG32450.0264.09098
TAGTAGA32700.0261.62089
TATTAGT32750.0261.221376
GGAGCGA24300.0260.401255
GGGGGGA34250.0259.686131
GGGAGCG24750.0255.070714
ATTAGTA33800.0253.542917
GGGGGAG35150.0253.45662
CGAAAGC25650.0248.996119
GGGAGCA10400.0238.269234
GCGAAAG26950.0236.985158
GAGCGAA27400.0230.939776
AGCGAAA27750.0229.621637
GCAAAAG16200.0155.694448
Produced by FastQC (version 0.11.2)