FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a16.3520000003f5b2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a16.3520000003f5b2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229077
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG189668.279312196335729No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA139136.073503669071972No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC66322.895096408631159No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA62692.7366344067715223No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG43251.8880114546637157No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT41201.7985218943848575No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT36721.6029544650925234No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA32031.3982198125521115No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA25221.1009398586501482No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA24951.089153428759762No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT24541.0712555167039903No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT22400.97783714646167Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT22330.9747814053789774No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA21140.922833806973201No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT12480.5447949816000733No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG11810.5155471740943002No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG11160.48717243546929634No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA7410.3234720203250436No Hit
GGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAA5590.24402275217503283No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT5540.2418400799731095No Hit
GGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGA5520.24096701109234012No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5510.24053047665195545No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT4190.18290793052117846No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG4110.17941565499810108No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAGCTGAAATGAG3380.14754864085001984No Hit
GGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAAC2530.11044321341732255No Hit
GAGAGGGTTCCTCATTATAGGTAAAGAAGACAGAAGATACGGACCAGCAT2300.10040292128847504No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA49400.0291.4171
GGGTTAT51150.0280.58162
GGTTATT51250.0278.30733
GTTATTA51250.0278.30734
TTATTAG51800.0275.067575
GGGGGGA40350.0274.52911
TTAGTAG52550.0271.422458
ATTAGTA52850.0269.602667
TATTAGT53000.0269.117926
GGAGCGA33700.0265.675055
GGGGAGC41650.0264.543823
GGGAGCG34200.0261.790924
CGAAAGC34500.0259.086949
GCGAAAG34650.0258.391058
TAGTAGA55800.0255.878149
GAGCGAA35100.0254.658116
AGCGAAA35350.0252.857137
GGGGGAG45800.0240.89522
GGGAGCA9300.0222.043014
CAAAAGC13050.0154.846749
Produced by FastQC (version 0.11.2)