FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a132.3520000003f58c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a132.3520000003f58c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225490
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC103714.599317042884385No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG64192.8466894319038536No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT57832.564637012727837No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA45292.008514790012861No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG35431.5712448445607343No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT35171.559714399751652No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT33391.4807752006740875Illumina Single End PCR Primer 1 (97% over 34bp)
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT29181.2940706904962527No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA27841.2346445518648277No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT18680.8284181116679232No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA18480.8195485387378598No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA16160.716661492749124No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT15600.6918266885449466No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG13310.5902700784957204No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA10500.46565257882832944No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8400.3725220630626635No Hit
GGGGGGAGCGAAAGCAGGTTTCATAGAAAATGGTTGGGAGGGAATGGTGG7470.3312785489378686No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7390.3277307197658433No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA4120.18271320235930638No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4090.1813827664197969No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT3880.17206971484323028No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA2550.11308705485830858No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG2350.10421748192824516No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA32900.0294.551671
GGGTTAT33500.0289.716432
GTTATTA33600.0287.53724
GGTTATT33800.0287.1453
TTATTAG34450.0280.442665
TTAGTAG34700.0277.99718
ATTAGTA35250.0274.496467
TATTAGT35350.0273.719946
CGAAAGC21750.0267.873579
GGGGAGC27000.0267.13893
TAGTAGA36100.0266.80759
GGAGCGA22000.0264.829565
GGGGGGA27400.0264.315671
GGGAGCG22400.0260.75894
GAGCGAA23250.0251.860216
GCGAAAG23250.0251.225818
AGCGAAA23350.0250.781597
GGGGGAG34950.0207.639482
GGGAGCA6750.0203.222234
CTAGGCG150.0028481842196.66666295
Produced by FastQC (version 0.11.2)