FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a12.3520000003f5cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a12.3520000003f5cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223962
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG137546.1412203856011285No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA101654.538716389387485No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC63332.827711843973531No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA48282.155722845839919No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG45442.0289156196140414No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT41721.86281601343085No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT29591.3212062760646897No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA26491.1827899375786963No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT26461.1814504246256061Illumina Single End PCR Primer 1 (97% over 34bp)
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT26181.168948303730097No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA26061.1635902519177361No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA24081.0751823970137793No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA22090.9863280377921255No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT17970.8023682589010637No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT15310.6835981103937275No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG14250.6362686527178718No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG13350.5960832641251641No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT7850.35050588939195043No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA6990.3121065180700297No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT5550.24780989632169742No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG5140.2295032192961306No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4550.20315946455202222No Hit
GGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAA4260.1902108393388164No Hit
GGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTGA4120.18395977889106188No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT2790.12457470463739385No Hit
GGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAAC2390.10671453192952376No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA43950.0290.63711
GGGTTAT45200.0282.599552
GTTATTA45250.0280.33154
GGTTATT45550.0279.132843
TTATTAG46050.0276.102085
TTAGTAG46750.0272.598948
TATTAGT46850.0271.702276
ATTAGTA47000.0270.521277
GGGGGGA36950.0269.451971
GGGGAGC37550.0265.146453
GGAGCGA29000.0258.37935
GGGAGCG29250.0257.683754
TAGTAGA49900.0255.095189
GCGAAAG29700.0251.29638
CGAAAGC30000.0248.291669
AGCGAAA30800.0243.27927
GAGCGAA31150.0240.072226
GGGGGAG41800.0238.54072
GGGAGCA10750.0220.906984
CAAAAGC14000.0166.464289
Produced by FastQC (version 0.11.2)