FastQCFastQC Report
Mon 7 Dec 2015
000000000-AKR50_l01n02_a1116.3520000003f68b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AKR50_l01n02_a1116.3520000003f68b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187430
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA91174.864215973963613No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC68553.6573654164221305No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG53572.8581337032492127No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG44722.385957424104999No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT42872.2872539081257No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG39722.1191911647014887No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA35411.8892386490956623No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC33891.808141706236995No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA27221.452275516192712No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA23211.2383289761510965No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT22711.2116523502107452No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT18640.9945046150562877Illumina Single End PCR Primer 1 (96% over 32bp)
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG16840.8984687616710239No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13900.7416102011417596No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA13670.7293389532091981No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG10260.5474043642960038No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG9170.4892493197460385No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA8630.4604385637304593No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6570.350530864856213No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA5100.27210158459158085No Hit
CGGGTTATTAGTAGAAACAAGGCAACTTCTTAGAGGGACAAGTGGCGTTC4280.2283519180494051No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4010.2139465400416155No Hit
CGGGTTATTAGTAGAAACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTT3810.20327588966547513No Hit
GGGGGGAGCGAAAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT3770.20114175959024702No Hit
CGGGTTATTAGTAGAAACAAGGGCAGGGACTTAATCAACGCAAGCTTATG3370.1798004588379662No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC3150.1680627434242117No Hit
GGGGGGAGCGAAAGCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACAC3130.16699567838659768No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT2880.15365736541642214No Hit
GGGGGGAGCGAAAGCAGGTGCATTGATTGAAGATCCAGATGAAGGCACAT2840.15152323534119405No Hit
GGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAAC2810.149922637784773No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA2520.13445019473936937No Hit
CGGGTTATTAGTAGAAACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTC2370.12644720695726405No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA2330.12431307688203597No Hit
GGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACA2320.12377954436322894No Hit
GGGGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGAC2110.1125753614682815No Hit
GGGGGGAGCAATTGGGACAGAAATTTGAGGAAATAAGATGGTTAATTGAA2100.11204182894947448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAG206.171285E-8295.08
CGGGTTA43650.0288.579621
GGGGGGA31300.0285.103851
GGGTTAT44900.0280.545652
GGTTATT45500.0276.5223
ATTAGTA45200.0274.76777
GTTATTA45650.0273.997834
GGGGGAG33150.0268.748142
TATTAGT46600.0268.09556
TAGTAGA46400.0267.343759
TTATTAG46950.0267.03945
CGAAAGC26150.0265.105169
TTAGTAG46900.0264.80818
GAGCGAA26150.0264.54116
GCGAAAG26300.0263.593148
GGAGCGA26250.0262.971445
GGGGAGC35250.0253.156023
GGGAGCG27600.0250.108694
AGCGAAA28400.0245.140847
GAGCAAA9000.0196.666666
Produced by FastQC (version 0.11.2)